In 1995, I began graduate studies on signal transduction by growth factors and
receptor tyrosine kinases in the laboratory of Graeme Guy at the Institute of Molecular and Cell Biology (IMCB) in Singapore, obtaining my PhD in 2000.
Sina Koch (Ehninger, TUD)-- «Aberrant subcellular localization as a potential mechanism contributing to the abnormal signaling of the mutant Flt3 - ITd
receptor tyrosine kinase in acute myeloid leukemia» (2007)
In addition to causing the functional inactivation of these phosphatases, low concentrations of H2O2 or an oxidative shift in the GSH: GSSH redox status strongly increases the activity of the basic insulin
receptor tyrosine kinase in the absence of insulin.
Not exact matches
The Janus
tyrosine kinases (Jaks) play a central role
in signaling through cytokine
receptors.
These
receptors, called
receptor tyrosine kinases (RTKs), transmit instructions through the cell wall and down through a cascade of reactions to a target gene
in the nucleus.
Among patients with advanced non-small cell lung cancer without a mutation of a certain gene (EGFR), conventional chemotherapy, compared with treatment using epidermal growth factor
receptor tyrosine kinase inhibitors, was associated with improvement
in survival without progression of the cancer, but not with overall survival, according to a study
in the April 9 issue of JAMA.
Epidermal growth factor
receptor (EGFR)
tyrosine kinase inhibitors (TKIs) are the preferred treatment option for patients with advanced non-small cell lung cancer (NSCLC) who have mutations
in the EGFR gene.
They found higher levels of JAK1
in resistant tumors, which caused increased expression of epidermal growth factor
receptor (EGFR)-- a
receptor tyrosine kinase that promotes cell proliferation.
PDGFRα is a cell surface
tyrosine kinase receptor involved
in organ development and tumor progression, it is present
in multiple cell types such as mesenchymal cells, neurons, astrocytes, megakaryocytes and oligodendrocyte progenitor.
Approximately 10 - 15 % of Caucasian and 30 - 35 % of Asian patients with NSCLC have a mutation
in the epidermal growth factor
receptor (EGFR), which can be successfully targeted with EGFR inhibitors called
tyrosine kinase inhibitors (TKI), such as erlotinib, gefitinib and afatinib.
And, indeed, previous research has shown that
receptor tyrosine kinases, e.g. insulin
receptors, and cytokine
receptors, e.g. growth hormone
receptors, exist
in dimeric form even
in the absence of ligands.
During the early years of my PhD studies, I was very fascinated by the exciting discoveries
in the field of signal transduction,
in particular how
receptor tyrosine kinases are activated to transmit their signals and how protein complexes are formed through defined protein folds (domains) interacting with specific cellular targets.
The researchers, including scientists from pharmaceutical company AstraZeneca, report
in an advanced online publication
in Nature Medicine on May 4, that their findings indicate «an underappreciated genomic heterogeneity»
in mechanisms of resistance to
tyrosine kinase inhibitor (TKI) drugs that target the Epidermal Growth Factor
Receptor (EGFR) mutation that drive some cases of non-small cell lung cancer (NSCLC).
Gene expression
in papillary thyroid carcinomas, with special reference to
tyrosine kinase receptors and growth factors.
In 2005, Cagan's team created a general fly model of a human thyroid tumor caused by mutations in the Ret receptor tyrosine kinase gene, then screened a panel of drugs including a kinase inhibitor called vandetanib that suppressed the tumor (Cancer Res, 65:3538 - 41, 2005
In 2005, Cagan's team created a general fly model of a human thyroid tumor caused by mutations
in the Ret receptor tyrosine kinase gene, then screened a panel of drugs including a kinase inhibitor called vandetanib that suppressed the tumor (Cancer Res, 65:3538 - 41, 2005
in the Ret
receptor tyrosine kinase gene, then screened a panel of drugs including a
kinase inhibitor called vandetanib that suppressed the tumor (Cancer Res, 65:3538 - 41, 2005).
We are focusing on a few key molecular pathways including; 1) Polycomb - mediated epigenetic gene silencing
in the tumor initiation, maintenance, and invasion, 2) c - Met (
receptor tyrosine kinase) signal transduction pathways
in stemness and migration of these tumor cells, 3) Novel mitogenic signaling pathways that are specific to GSCs, and 4) Identification of radio - and chemo - sensitizing pathway to maximize therapeutic efficacy.
These events occur when specific extracellular molecules bind to
receptor proteins
in the plasma membrane known as
receptor tyrosine kinases and heterotrimeric G - protein - coupled
receptors.
Expression of the
receptor tyrosine kinase Tie2
in neoplastic glial cells is associated with integrin beta1 - dependent adhesion to the extracellular matrix.
AXL encodes a
receptor tyrosine kinase that promotes breast cancer bone metastasis
in mouse models (41).
Further, we identified a role for ABL
kinases in promoting the expression of multiple pro — bone metastasis genes such as AXL (which encodes a
receptor tyrosine kinase), IL6 (which encodes interleukin - 6), MMP1 (which encodes matrix metalloproteinase 1), and TNC (which encodes tenascin - C) through TAZ - and signal transducer and activator of transcription 5 (STAT5)-- mediated signaling.
Also, genes that code for
receptor tyrosine kinases, a family of
receptors on the surface of cells, may rearrange to form multiple distinct gene fusion partners, as evidenced
in an article by Kulkarni, et al, on a translational study involving a patient who developed a BRAF fusion following treatment with a BRAF inhibitor1.
These mutant
kinases are attractive therapeutic targets, as demonstrated by the efficacy of imatinib
in BCR - ABL — positive chronic myelogenous leukemia (CML), 5 as well as in MPD associated with activating alleles involving PDGFRA or PDGFRB.2, 6,7 In addition, activating mutations in the FLT3 receptor tyrosine kinase are the most common genetic event in acute myeloid leukemia (AML), and specific inhibitors of the FMS - like tyrosine kinase 3 (FLT3) have entered late - stage clinical trials.8 Although mutations in tyrosine kinases and in other genes have been identified in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in BCR - ABL — positive chronic myelogenous leukemia (CML), 5 as well as
in MPD associated with activating alleles involving PDGFRA or PDGFRB.2, 6,7 In addition, activating mutations in the FLT3 receptor tyrosine kinase are the most common genetic event in acute myeloid leukemia (AML), and specific inhibitors of the FMS - like tyrosine kinase 3 (FLT3) have entered late - stage clinical trials.8 Although mutations in tyrosine kinases and in other genes have been identified in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in MPD associated with activating alleles involving PDGFRA or PDGFRB.2, 6,7
In addition, activating mutations in the FLT3 receptor tyrosine kinase are the most common genetic event in acute myeloid leukemia (AML), and specific inhibitors of the FMS - like tyrosine kinase 3 (FLT3) have entered late - stage clinical trials.8 Although mutations in tyrosine kinases and in other genes have been identified in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
In addition, activating mutations
in the FLT3 receptor tyrosine kinase are the most common genetic event in acute myeloid leukemia (AML), and specific inhibitors of the FMS - like tyrosine kinase 3 (FLT3) have entered late - stage clinical trials.8 Although mutations in tyrosine kinases and in other genes have been identified in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in the FLT3
receptor tyrosine kinase are the most common genetic event
in acute myeloid leukemia (AML), and specific inhibitors of the FMS - like tyrosine kinase 3 (FLT3) have entered late - stage clinical trials.8 Although mutations in tyrosine kinases and in other genes have been identified in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in acute myeloid leukemia (AML), and specific inhibitors of the FMS - like
tyrosine kinase 3 (FLT3) have entered late - stage clinical trials.8 Although mutations
in tyrosine kinases and in other genes have been identified in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in tyrosine kinases and
in other genes have been identified in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in other genes have been identified
in a subset of MPD and AML, in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in a subset of MPD and AML,
in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknow
in many cases the genetic events that contribute to the molecular pathogenesis of these diseases remain unknown.
In 2005, the identification of an activating mutation in JAK2 (the V617F mutation) as a STAT5 - activating and disease - causing genetic alteration in a significant proportion of patients with myeloproliferative neoplasms (MPNs) has emphasized the oncogenic role of the JAK tyrosine kinases in hematologic malignancies.2 — 5 JAK2 is a member of the Janus tyrosine kinase family comprising three other mammalian non-receptor tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine receptors lacking intrinsic kinase activity to mediate cytokine - induced signal transduction and activation of STAT transcription factors.6 All JAKs share a similar protein structure and contain a tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.
In 2005, the identification of an activating mutation
in JAK2 (the V617F mutation) as a STAT5 - activating and disease - causing genetic alteration in a significant proportion of patients with myeloproliferative neoplasms (MPNs) has emphasized the oncogenic role of the JAK tyrosine kinases in hematologic malignancies.2 — 5 JAK2 is a member of the Janus tyrosine kinase family comprising three other mammalian non-receptor tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine receptors lacking intrinsic kinase activity to mediate cytokine - induced signal transduction and activation of STAT transcription factors.6 All JAKs share a similar protein structure and contain a tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.
in JAK2 (the V617F mutation) as a STAT5 - activating and disease - causing genetic alteration
in a significant proportion of patients with myeloproliferative neoplasms (MPNs) has emphasized the oncogenic role of the JAK tyrosine kinases in hematologic malignancies.2 — 5 JAK2 is a member of the Janus tyrosine kinase family comprising three other mammalian non-receptor tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine receptors lacking intrinsic kinase activity to mediate cytokine - induced signal transduction and activation of STAT transcription factors.6 All JAKs share a similar protein structure and contain a tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.
in a significant proportion of patients with myeloproliferative neoplasms (MPNs) has emphasized the oncogenic role of the JAK
tyrosine kinases in hematologic malignancies.2 — 5 JAK2 is a member of the Janus tyrosine kinase family comprising three other mammalian non-receptor tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine receptors lacking intrinsic kinase activity to mediate cytokine - induced signal transduction and activation of STAT transcription factors.6 All JAKs share a similar protein structure and contain a tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.
in hematologic malignancies.2 — 5 JAK2 is a member of the Janus
tyrosine kinase family comprising three other mammalian non-receptor
tyrosine kinases (JAK1, JAK3 and TYK2) that associate with cytokine
receptors lacking intrinsic
kinase activity to mediate cytokine - induced signal transduction and activation of STAT transcription factors.6 All JAKs share a similar protein structure and contain a
tyrosine kinase domain at the C - terminus flanked by a catalytically inactive pseudokinase domain with
kinase - regulatory activity, by an atypical SH2 domain and by a FERM domain that mediates association to the membrane - proximal region of the cytokine
receptors.7, 8 Soon after the discovery of JAK2 V617F, we and others described that activating JAK1 mutations are relatively common
in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.
in adult patients with T - cell acute lymphoblastic leukemia (ALL) and participate
in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported in AML and breast cancer patients.
in ALL development allowing for constitutive activation of STAT5.9 — 11 Several STAT5 - activating JAK1 mutations were also reported
in AML and breast cancer patients.
in AML and breast cancer patients.10
In addition, at least some Eph
receptors can also signal through non-canonical mechanisms that are independent of ligand binding and
kinase activity, for example through interplay with other
receptor tyrosine kinase families and with serine / threonine
kinases.
Activated JAK
kinases in turn phosphorylate - specific
tyrosine motifs found
in receptor domains, which then recruit the specific monomeric STATs to the
receptor complex.
For example, our past work showed that two conserved
tyrosine phosphorylation sites
in the juxtamembrane segment of the Eph
receptors not only mediate association with binding partners but also regulate
receptor kinase activity.
They found that certain GBM tumors — particularly those deficient
in PTEN — had multiple co-activated
receptor tyrosine kinases (RTKs).
Twelve - hour exposure of 3T3 - L1 adipocytes to H (2) O (2) or TNF - alpha resulted
in the increase of c - Jun NH (2)- terminal
kinase (JNK) activation and insulin
receptor substrate 1 (IRS1) serine 307 phosphorylation, concomitantly with the decrease
in insulin - stimulated IRS1
tyrosine phosphorylation and cellular glucose uptake.
METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization;
tyrosine phosphorylation of the insulin
receptor or of the insulin
receptor substrate - 1
in cell lysates; association of phosphatidylinositol 3 -
kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin
receptor substrate - 1 using a
in vitro
kinase assay; association of the p85 subunit of phosphatidylinositol 3 -
kinase with phosphotyrosine proteins or with insulin
receptor substrate - 1; and
in vitro activity of immunoprecipitated Akt1.
«
In the near future, we'll likely see more medications specifically targeting receptors on cells involved in allergic reactions, such as tyrosine kinase inhibitors (mast cells), for dermatologic use.&raqu
In the near future, we'll likely see more medications specifically targeting
receptors on cells involved
in allergic reactions, such as tyrosine kinase inhibitors (mast cells), for dermatologic use.&raqu
in allergic reactions, such as
tyrosine kinase inhibitors (mast cells), for dermatologic use.»
Investigated TAM family
receptor tyrosine kinase gene expression
in response to small molecule inhibitors
in glioblastoma multiforme