Sentences with phrase «recognizes pam»
The most commonly used form of Cas9, derived from the bacteria Streptococcus pyogenes and known as SpCas9, recognizes PAM sequences in which any nucleotide is followed by two guanine DNA bases.
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To get around this limitation the MGH team set up an engineering system that allowed them to rapidly evolve the ability of SpCas9 to recognize different PAM sequences.
From a collection of randomly mutated SpCas9 variants, they identified combinations of mutations that enabled SpCas9 to recognize new PAM sequences.