Sentences with phrase «recombinant human fibroblast»
The conventional hESC medium contained 80 % (vol / vol) KnockOut Dulbecco's Modified Eagle's Medium (DMEM) and 20 % (vol / vol) KnockOut serum replacement supplemented with 2 mM Glutamax, 0.1 mM β - mercaptoethanol, 0.1 mM MEM non-essential amino acids (Cambrex Bio Science, Walkersville, Inc., Walkersville, MD), 50 U / ml penicillin - 50 μg / ml streptomycin (Cambrex Bio Science) and 8 ng / ml recombinant human fibroblast growth factor (bFGF, R&D Systems, Minneapolis, MN).
https://biomedical-engineering-online.biomedcentral.com/ Not exact matches
and cultured in 5 % O2 / 7 % CO2 in Bottenstein - Sato F12 medium with 10 ng / ml human recombinant basic fibroblast growth factor (Peprotech) on a substrate of 1 µg / cm2 fibronectin (Chemicon) and 0.5 µg / cm2 laminin (Invitrogen).