Sentences with phrase «recombination gene targeting»

Not exact matches

Not least the fact that gene targeting using homologous recombination or any other method is not perfect — the added DNA is sometimes inserted into the wrong place in the genome, and the process can trigger other kinds of mutations too.

The marriage of Professors Capecchi and Smithies» homologous recombination technique with Sir Martin's stem cell discoveries has created the highly versatile new technology of gene targeting.

from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA

In that year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA.

Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre — loxP — mediated gene targeting.

This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.

(A) The CCHa2 - RKO51 - 2 allele was generated by gene targeting using homologous recombination.

This approach allows to obtain mouse models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.

CCHa2 - RKO51 - 2 was generated by gene targeting using homologous recombination [22][23].

CCHa2 - RKO51 - 2 and CCHa2 - RTAL - 34 are putative null alleles, which were generated by gene targeting using homologous recombination and TALEN, respectively (see below).

Gene targeting by homologous recombination in hiPS cell has proven difficult.

In heritable recombination, gene modifications (blue and green) are incorporated into the target gene (yellow).

He completed his postdoctoral studies in gene targeting and DNA recombination at Massachusetts Institute of Technology (MIT).

We have generated new genetic tools for the analysis of in vivo mast cell functions that allow for cell type - specific inactivation («knock out «-RRB- of target genes selectively in mast cells by Cre loxP - mediated recombination.

Microsatellite markers can become separated from the target gene during recombination.