The sequences of
the reconstructed gene models for OR genes in FASTA format.
The reconstructed gene models for OR genes based on the OM RNAseq dataset, provided in GTF format.
The median length of Ensembl V2R genes is 2,559 nt, while for the V2R
reconstructed gene models it is 2,912 nt (Figure 7C).
The reconstructed gene models for VR genes based on the VNO RNAseq dataset, provided in GTF format.
Not exact matches
If the work proceeds as expected, «all interactions between
genes, proteins, and small molecules will be revealed, and the whole cellular network will be
reconstructed,» says Igor Goryanin, who heads cell
modeling at GlaxoSmithKline in Stevenage, U.K.
When we compare the complete
gene models we have
reconstructed with those currently annotated, both the amount of the receptor transcript sequence, and the proportion that is unique between receptors increases substantially.
All
genes with a lipocalin domain (IPR000566) were extracted from Ensembl version 68 and a phylogeny was
reconstructed in MEGA using the neighbour - joining method with the Kimura - 2 parameter
model of substitution [55].
We next compared the 5 ′ ends of the OR
gene models reconstructed here using Cufflinks, to the proposed transcription start sites (TSS) reported by Plessy et al. (2012) using nanoCAGE [29].
We similarly compared the 5 ′ ends in our
reconstructed models to these data and found that a third of the receptor
genes differ in less than 100 nucleotides and 80 % of the data is contained within a 1.5 kb window (Figure S8).
We next calculated the length for each receptor
gene based on the existing Ensembl and our new
reconstructed models.