For the other experiments, recombinant HA (His - tagged) was immobilized at 5 µl / min − 1 on NTA sensor chips with a target density of 350 response units, and the antibodies at concentrations ranging from 1 to 30 nM in HBS - P buffer were injected at 20 µl / min − 1 over the immobilized recombinant HA or
reference cell surface, followed by a 600s dissociation phase.
For kinetic analysis, injections over
reference cell surface and injections with buffer were subtracted from the data.
EM4CO4 and SF1009 - 3FO1 antibodies were immobilized at 10 µl / min − 1 on a CM5 sensor chip by amine coupling and recombinant HA at concentrations ranging from 0.5 to 15 nM in HBS - EP buffer were injected at 20 µl / min − 1 over the immobilized antibodies or
reference cell surface.
Not exact matches
The Grieneisen lab uses the
reference plant Arabidopsis and computer models to focus on the misshapen misfits called pavement
cells that make up the
surface of plant leaves.