Sentences with phrase «rejected allografts»

As controls, syngeneic grafts at 100 days post-transplantation and acutely rejected allografts harvested 13 days post-transplantation were examined after H & E staining (Figure 6).

As expected, acutely rejected allografts were massively infiltrated by mononuclear cells, whereas tolerant allografts were free of infiltrating cells at both 40 and 100 days post-transplantation.

In contrast, none of the syngeneic or rejected allografts showed any FoxP3 - expressing cells, as reflected by the merged stains of both FoxP3 and CD4 stains in F and G, respectively.

In a non-human primate study, Haanstra et al. reported that within the graft - infiltrating lymphocytes, accepted allografts did not have greater numbers of Tregs than rejecting allografts [22].

Not exact matches

Interestingly, recipients of CD8 + T lymphocytes failed to mount DTH responses to BALB / c alloantigens (Figure 4), even though similar panels of mice rejected 95 % of their BALB / c corneal allografts (Figure 2B).

Immunohistochemical staining of rejected human corneal allografts has revealed the presence of apoptosis in corneal stromal cells (5).

Spleen cell suspensions were collected 7 — 14 days after CD4 KO mice rejected BALB / c corneal allografts.

By contrast, 71 % (5/7) of the beige nude mice that received spleen cells from CD4 KO mice rejected their BALB / c corneal allografts.

The present demonstration of T cell - mediated apoptosis of allogeneic corneal cells from CD4 KO mice is consistent with previous findings, which noted the presence of apoptotic keratocytes and corneal endothelial cells in rejected corneal allografts in humans and rats respectively (5, 32).

Spleen cells were collected from CD4 KO mice 7 — 14 days after they had rejected BALB / c corneal allografts.

T lymphocytes are required for the rejection of corneal allografts, as athymic, T cell - deficient nude mice do not reject corneal allografts (3).

The role of DN T cells in corneal allograft rejection was confirmed in two separate in vitro assays in which CD8 − cells were isolated from CD4 KO donors that had rejected corneal allografts and were found to induce apoptosis of donor - specific corneal cells.

The CD8 + T cells were already primed, as they were collected from CD4 KO mice that had rejected corneal allografts and thus, may have functioned differently from CD8 + T cells from naïve CD4 KO mice.

Accordingly, spleens were collected from either naïve C57BL / 6 CD4 KO mice or CD4 KO mice that had rejected BALB / c corneal allografts 7 to 14 days earlier.

Indeed, our results indicate that adoptive transfer of CD8 + T cells from CD4 KO mice that had rejected corneal allografts resulted in the rejection of 95 % of the corneal allografts transplanted to athymic recipients.

Histopathological examination of rejected corneal allografts in CD4 KO mice revealed a mixed inflammatory infiltrate containing large numbers of neutrophils and mononuclear cells, which was indistinguishable from the infiltrate seen in rejected corneal allografts in wild - type mice (data not shown).

However, in vitro assays using spleen cells from CD4 KO mice that had rejected BALB / c corneal allografts failed to detect CTL activity against donor corneal epithelial or endothelial cells.

The results indicated that 54 % (38/70) of the BALB / c corneal allografts underwent rejection in the CD4 KO hosts, while 100 % of the grafts were rejected in wild - type C57BL / 6 mice (Figure 1).

None of the ten C57BL / 6 beige nude mice rejected BALB / c corneal allografts (Figure 2A).

BALB / c corneal allografts were transplanted to C57BL / 6 beige nude mice that received either CD8 − or CD8 + T cells from C57BL / 6 CD4 knockout (KO) mice that had rejected BALB / c corneal allografts.

Spleen cells were isolated 7 — 14 days after C57BL / 6 CD4 KO mice had rejected BALB / c corneal allografts and were tested for anti - BALB / c CTL in a conventional 4 - hr 51Cr - release assay using BALB / c corneal epithelial and endothelial target cells.

Moreover, we have recently shown that interferon - γ (IFN - γ) KO mice, which can not mount classical Th1 immune responses, are nonetheless capable of rejecting corneal allografts (18, 19).

Balb / c third - party allografts were acutely rejected in chimeric animals, as were control MRL allografts in non-chimeric animals.