Sentences with phrase «reporter constructs»

Once they'd found suitable pairs, they constructed three components: GBPa - VP16 (activation domain); GBPb - GAL4 (DNA - binding domain); and a UAS - driven luciferase reporter construct.

The mutant reporter constructs pXP2 - mut - 1, pXP2 - mut - 2, and pXP2 - mut - 3 were generated from pXP2 (− 310) by Mutagenesis kit.

Although this problem can, to some extent, be addressed by the use of reporter constructs (e.g. Diptericin - LacZ) that provide quantitative or visual read - outs from specific immune response genes, we still lack the ability to follow bacterial infections in real - time in the critical early stages of infection.

The results suggested that the levels of activation induced by DDX3 of the luciferase activity of serial reporter constructs from (− 1,002 / +8) p21 - Luc to (− 123 / +8) p21 - Luc were comparable with the level of activation elicited by the full - length promoter - driven reporter p21 - Luc (5 - to 10-fold; Fig. 3B and Supplementary Fig.

The fold transactivation of the various p21waf1 / cip1 promoter — directed reporter constructs by DDX3 is shown as fold activation relative to the control transfection and is derived from at lease three independent experiments done in triplicate.

To expand our repertoire of tools to investigate cellular changes that occur during DENV infection, we are creating additional reporter constructs for detection of DENV infection of living cells.

Sorting breast cancer cells using a new reporter construct allows the interrogation of a highly pure population of cancer stem cells and may allow for the discovery of new therapeutic targets

The LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection of host cells.

To this end, we have developed a reporter construct that generates a fluorescent signal specifically in cells infected with dengue (DENV).

A, pTER - si - IRF - 1 was transfected into U3A cells with the reporter construct pXP2 (− 310) and the indicated expression plasmids.

reporter construct.

Moving to an in vivo system, Tang et al. electroporated T - DDOGs, GFP, and a reporter construct into the mouse retina.

A reporter constructing a story has various puzzle pieces to connect.