Sentences with phrase «reporter gene construct»

Luciferase activity was shown relative to the values obtained in the cells transfected with the same reporter gene construct and empty vectors.

The reporter gene construct pXP2 (− 310) was cotransfected with indicated expression plasmids into STAT1 - null U3A cells (C) or IRF - 9 — deficient U2A cells (D).

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instruction.

D, different reporter gene constructs were, respectively, cotransfected with IRF - 1 or IRF - 9 / STAT2 into U3A cells, as indicated.

C, schematic representation of the wt or mutant RIG - G promoter - luciferase reporter gene constructs.

Not exact matches

Although this problem can, to some extent, be addressed by the use of reporter constructs (e.g. Diptericin - LacZ) that provide quantitative or visual read - outs from specific immune response genes, we still lack the ability to follow bacterial infections in real - time in the critical early stages of infection.

«This prototype paves the way for constructing a family of reporter genes, each with proteins tailored to have a specific radiofrequency response,» says MRI researcher Assaf Gilad, Ph.D., lead author of the study.

Labs have successfully utilized 2A and IRES elements within the same multicistronic vector, effectively making a construct that expresses multiple unique genes at equivalent levels upstream of an IRES fluorescent reporter for easy detection.5

The GENSAT project is designed to document the expression patterns of all genes in the nervous system and to generate transgenic mice expressing reporter constructs that recapitulate the authentic expression patterns of selected genes.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS