Sentences with phrase «reporter genes for»

The Nature study, titled «Acoustic reporter genes for noninvasive imaging of microbes in mammalian hosts,» was funded by the National Institutes of Health, the Canadian Institute of Health Research, the Burroughs Wellcome Fund, the Packard Fellowship, the Pew Scholarship, the Heritage Medical Research Institute, the National Science Foundation, and the Natural Sciences and Engineering Research Council of Canada.

«This is the first acoustic reporter gene for use in ultrasound imaging,» says Shapiro.

«An effective reporter gene for MRI is a «holy grail» in biomedical imaging because it would allow cellular function to be observed non-invasively,» says Shapiro.

Research Paper The human somatostatin receptor type 2 as an imaging and suicide reporter gene for pluripotent stem cell - derived therapy of myocardial infarction Katrien Neyrinck, Natacha Breuls, Bryan Holvoet, Wouter Oosterlinck, Esther Wolfs, Hubert Vanbilloen, Olivier Gheysens, Robin Duelen, Willy Gsell, Ivo Lambrichts, Uwe Himmelreich, Catherine M. Verfaillie, Maurilio Sampaolesi, Christophe M. Deroose Theranostics 2018; 8 (10): 2799 - 2813.

Not exact matches

Bloomberg News Reporter Shruti Singh moderated a discussion of six panelists: Bruce Friedrich, executive director of The Good Food Institute; Angela Mason, associate vice president of Windy City Harvest; Karen Lehman, director of Fresh Taste; Gene Bauer, president and co-founder of Farm Sanctuary; Behtash Bahador of the Barilla Center for Food & Nutrition Alumni Association; and John Steven Bianucci, director of impact at Iroquois Valley Farms.

In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.

This is how he came to develop green fluorescent protein as a reporter for gene expression, for which he was coawarded a 2008 Chemistry Nobel Prize.

In this new study, researchers employed a cell - based reporter system to screen a collection of over 1000 gene - regulatory factors called microRNAs for the ability to influence this transition and identified some with particularly potent effects.

Imaging techniques that rely on light — such as taking pictures of cells tagged with a «reporter gene» that codes for green fluorescent protein — only work in tissue samples removed from the body.

When the reporter for Nanog is inserted upstream of the gene, the reporter does not faithfully represent the gene's activity (lower left, green), compared with a reporter inserted downstream of the gene (lower right, red).

For other genes, they might be more resistant to this type of disturbances caused by a reporter.»

Then for HARE5, the most active enhancer in an area of the brain called the cortex, they made minigenes containing either the chimp or human version of the enhancer linked to a «reporter» gene that caused the developing mouse embryo to turn blue wherever the enhancer turned the gene on.

Together, these reporter gene experiments confirmed the expression of Tie2 by pericytes and further validated Ng2 - Cre as a robust pan-pericyte driver for in vivo mutagenesis studies.

«This prototype paves the way for constructing a family of reporter genes, each with proteins tailored to have a specific radiofrequency response,» says MRI researcher Assaf Gilad, Ph.D., lead author of the study.

Labs have successfully utilized 2A and IRES elements within the same multicistronic vector, effectively making a construct that expresses multiple unique genes at equivalent levels upstream of an IRES fluorescent reporter for easy detection.5

Dr. Shendure's research group in Seattle pioneered exome sequencing and its earliest applications to gene discovery for Mendelian disorders (e.g. Miller and Kabuki syndrome) and autism; cell - free DNA diagnostics for cancer and reproductive medicine; massively parallel reporter assays and saturation genome editing; whole organism lineage tracing; and massively parallel molecular profiling of single cells.

These reporters allow you to easily screen or select for cells that are expressing your gene of interest at high levels.

(A and B) NFκB gene reporter assay determination of inhibition of MDP - stimulated NFκB activity with RIPK2 inhibitors (A) and an approximate IC50 determination for inhibition of MDP driven - NFκB activation using RIPK2 inhibitors 1 and 2 (B).

For the co-expression of multiple unique genes, you can start with a plasmid that has multiple cloning sites flanking the multicistronic element (s), or you could replace one of the reporter genes above with your gene or genes of interest.

Hygromycin selection was maintained for only a week, but transgene expression from the plasmid carrying the EGFP reporter gene suggested that the plasmid remained in the cells for another week before being eliminated (Figure S1).

Gene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteratGene expression Reporter genes, such as luciferase or GFP, can also be assessed in microplate readers, enabling in vitro and in vivo determination of gene expression for studies using markers of genetic alteratgene expression for studies using markers of genetic alteration.

In this approach, a promoterless reporter gene (for instance encoding beta galactosidase, or beta geo) is introduced into ES cells.

According to the Hollywood Reporter, Channing would play a «Gene Kelly - type» star, while Ralph is in line for the part of Laurence Lorenz, a studio director, and Tilda is eyeing the role of a powerful Hollywood gossip columnist.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS