Sentences with phrase «responder splenocytes»
An equal number of MRL responder splenocytes were added and the cells cultured for 48 h before pulsing.
http://journals.plos.org/ Not exact matches
Additionally, splenocytes of recipient animals were used as responder cells in mixed lymphocyte reactions and ELISPOT to determine responses to donor alloantigen.
In addition, mixed lymphocyte cultures using the cells of the chimeric animals as responder cells to donor splenocytes failed to demonstrate any sensitization or clonal deletion of alloreactive T cells; i.e. T cell responses were identical to those of control animals (n = 4)(data not shown).
Balb / c splenocytes were used as third - party responder cells assuming that these would induce the same degree of T cell proliferation in tolerant and control animals.
One of the assays widely used in the literature is the application of splenocytes from tolerant animals as responder cells in a 4 - day mixed lymphocyte culture.