Sentences with phrase «restriction endonuclease»

Briefly, randomly amplified libraries were cleaved with a Type IIs restriction endonuclease (GsuI) and truncated adapters were ligated on the resulting strand ends.

In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end - labeled oligonucleotide probe hybridized in solution to the amplified beta - globin sequences.

«And given the state of the chimeric restriction endonuclease art prior to the patent's 1999 date, there are some serious doubts as to its validity.

Before in vitro transcription, we linearized the plasmids with appropriate restriction endonucleases and generated sense and antisense riboprobes using in vitro transcriptions (Roche Applied Science) with T7 polymerase and the DIG RNA labeling kit (Roche Diagnostics).

He cited the example of the Hamilton Smith, who was working in the obscure field of bacterial immunity and discovered restriction endonucleases, which revolutionized DNA modification and earned a Nobel Prize.

Within a few years, the type II restriction endonucleases became widely used tools in fundamental and applied research on genetic information at the molecular level.

Not exact matches

This is called restriction and the endonuclease in question is the restriction enzyme.