Sentences with phrase «restriction fragment length polymorphism»

Martin and Palumbi did this, using a technique known as restriction fragment length polymorphism analysis.
The plasmids targeting each gene were respectively transfected into goat fibroblasts, and their genome modification efficiency was determined at day 3 using restriction fragment length polymorphism (RFLP) assay (see Methods).
Gale and his colleagues were the first to develop restriction fragment length polymorphisms for analysing the genomes of cereal crops, and have built on that expertise to construct detailed genetic maps of barley, rye and pearl millet, as well as wheat.
By the 1990s, DNA testing had advanced somewhat through the use of restriction fragment length polymorphism, but that technique required more material than was available.
Is the Subject Area «Restriction fragment length polymorphism analysis» applicable to this article?
Site - specific mutations will make the target locus resistant to the restriction enzyme treatment (uncut), which can be detected by restriction fragment length polymorphism (RFLP) assay.
They rotate among several different areas for 1 month each: microanalysis, PCR (polymerase chain reaction), and RFLP (restriction fragment length polymorphism).
Abbreviations: CFU, colony forming unit; CGH, comparative genome hybridization; CHEF, contour - clamped homogenous electric field; Chr, Chromosome; DSBs, double strand breaks; FACS, fluorescence - activating cell sorting; FLC, fluconazole; NGS, next generation sequencing; NS, niger seed; PFGE, pulsed field gel electrophoresis; RFLP, restriction fragment length polymorphism; SNP, single - nucleotide polymorphism; TS, temperature sensitive
In this study, we used terminal - restriction fragment length polymorphism and 16S rRNA gene sequencing approaches to characterize the bacterial composition of the colonic microflora in a patient suffering from recurrent CDAD before and after treatment by fecal transplantation from a healthy donor.
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