Gale and his colleagues were the first to
develop restriction fragment length polymorphisms for analysing the genomes of cereal crops, and have built on that expertise to construct detailed genetic maps of barley, rye and pearl millet, as well as wheat.
They rotate among several different areas for 1 month each: microanalysis, PCR (polymerase chain reaction), and RFLP (
restriction fragment length polymorphism).
Abbreviations: CFU, colony forming unit; CGH, comparative genome hybridization; CHEF, contour - clamped homogenous electric field; Chr, Chromosome; DSBs, double strand breaks; FACS, fluorescence - activating cell sorting; FLC, fluconazole; NGS, next generation sequencing; NS, niger seed; PFGE, pulsed field gel electrophoresis; RFLP,
restriction fragment length polymorphism; SNP, single - nucleotide polymorphism; TS, temperature sensitive
In this study, we used terminal -
restriction fragment length polymorphism and 16S rRNA gene sequencing approaches to characterize the bacterial composition of the colonic microflora in a patient suffering from recurrent CDAD before and after treatment by fecal transplantation from a healthy donor.