Since completing the initial paper published Thursday, the team has analyzed 50 more samples from eye fluid
of retinoblastoma patients, including those with less aggressive tumors whose eyes were saved, to get a start on determining what factors might be at play in tumor progression.
The cyclin - dependent kinase (CDK) inhibitors p21waf1 and p27kip1 and underphosphorylated
retinoblastoma protein levels were up - regulated and cyclin A levels were decreased in a concentration - dependent manner (Fig. 5B and C).
Under the mentorship of Drs. Richard L. Hurwitz and Catherine M. Bollard, he characterized the immune response against an adenoviral vector delivered intravitreally
in retinoblastoma patients.
And it's too early to say how or even whether CNVs contribute to the risk of a mutated
retinoblastoma gene.
Dr. Jesse Berry, 36, has long been frustrated by not being able to tell the parents of her young
retinoblastoma patients what to expect.
Standard chemotherapy agents don't work specifically on blocking
retinoblastoma tumors but instead broadly inhibit DNA replication.
Michael Dyer, a Ph.D. biologist who studies the childhood eye
cancer retinoblastoma at St. Jude Children's Research Hospital in Memphis, Tennessee, says a day with patients back in 2004 was the «single most important event» in his career.
Drs. Jonathan Kim (left) and Jesse Berry, both pediatric ocular oncologists, collaborated on developing a liquid biopsy for children
with retinoblastoma.
Dyer asked the clinicians if the 1986 discovery of the gene responsible
for retinoblastoma — a milestone discovery in basic research, as it was the first human - disease gene to be cloned — had made a difference in the lives of the patients.
«It won't cure children on its own, but it is a step toward saving eyes,» said Dr. Brenda Gallie, an expert on the genetics of
retinoblastoma at the Hospital for Sick Children in Toronto.
Western blot analysis following treatment with rapamycin (B) or transient transfection with mTOR siRNA (C) showed increased levels of the CDK inhibitors p21waf1 and p27kip1,
underphosphorylated retinoblastoma protein, and decreased cyclin A in a dose - dependent manner.
«We made the surprising discovery that a protein
called Retinoblastoma (Rb) is present in adult neurons,» explains Zochodne.
Damian Salgado, pictured in May 2017 at SeaQuest Aquarium in Las Vegas, lost his right eye
from retinoblastoma.
Knudsen and colleagues showed that tumors
whose retinoblastoma (RB) gene no longer worked were likely to become hormone resistant, but remarkably were more likely to respond to cabazitaxel.
This insight will allow us to focus on that stage to better understand
how retinoblastomas originate.»
Mapping the epigenetic changes in developing
retinoblastoma mouse and human cells yielded similar important insights, Dyer said.
Enhanced SOX2 expression in
retinoblastoma tissues and peripheral blood is associated with the clinicopathological characteristics of the disease.
The paper «MYC Releases Early Reprogrammed Human Cells from Proliferation Pause
via Retinoblastoma Protein Inhibition» was published by Cell Reports on April 10, 2018.
Incorporating topotecan into front - line therapy of advanced
localized retinoblastoma cures patients and improves survival of eyes with useful vision.
In January, TGI and its collaborators published three Nature papers that came online the same day: one on the genetic basis of an aggressive pediatric leukemia, another on genomic and epigenetic analyses of
childhood retinoblastoma, and a third on tumor evolution in relapsed AML.
In many developing countries where tumors can progress until they literally burst out of eyes,
retinoblastoma remains an often fatal diagnosis.
Outside retinoblastoma experts say they're excited about the finding because they've never been able to grab samples of the tumors they treat without removing the eye.
Now, because a young oncologist at Children's Hospital Los Angeles followed a hunch, there may be a safe, non-invasive way to
biopsy retinoblastoma tumors — using fluid that's removed from the eye during chemotherapy and typically discarded.
She and her husband went from their son's pediatrician in Las Vegas to an ophthalmologist and then to a retinal specialist and finally to the LA children's hospital, which is one of just a handful in North America that specialize in
retinoblastoma treatment.
«Most people assumed that
retinoblastoma genes were inherited in a dominant fashion — that is, if you had the gene, you would get the cancer,» Knudson said.
Now, preliminary work reported here today at the American Society of Human Genetics meeting suggests that men who have more CNVs than average may be more likely to sire children with the eye
cancer retinoblastoma.
Knudson, who has been affiliated with the Fox Chase Cancer Center in Philadelphia since 1976, was studying children
with retinoblastoma, a cancer of the eye, noting differences between the 40 percent of cases with heritable tumors and the 60 percent of non-heritable cases.
The researchers found that
retinoblastoma protein (RB), a protein known to reduce cancer risk, is significantly less active in male brain cells than in female brain cells.