Forty such lines were tested for their ability to increase aggression when
their labeled neurons were activated.
Researchers have good methods for
labeling neurons based on where in the brain they developed, but the methods for
labeling neurons that develop at the same time are less precise.
Photo of a living Brainbow zebrafish, taken by Zachary Tobias (a research technician in Weissman - Unni's lab), showing a brightly
labeled neuron with its cell body (white) at bottom.
This image shows fluorescently
labeled neurons, overlaid with an illustration of a person scratching.
Such vectors have become critical tools for delivering genes to treat disease or to
label neurons and their connective fibers with fluorescent colors to map out their locations.
Basically, you need a way of
labeling neurons that were active during a specific experience, and a switch to operate them.
Accurately
labelling neurons with markers such as fluorescence, he says, will probably be the key challenge in the eventual goal of creating a «census» of different cell types in the brain.
Sandia National Laboratories researchers are drawing inspiration from neurons in the brain, such as these green fluorescent protein -
labeled neurons in a mouse neocortex, with the aim of developing neuro - inspired computing systems to reboot computing.
Finally, hydroxystilbamadine, also known as fluoro - gold, is a dye that can be used as a retrograde enhancer for
labelling neurons by injecting it in vivo.
He used an assortment of anatomical tracing techniques to
label neurons in their entirety — a cell body with a long axon extending out in one direction and the branched tendrils of dendrites protruding from the other.
Labeled neurons cover lateral and ventral parts of the DLL and are found in the SpRT and LdOPT (compare Fig. 1C).
The distribution of the few retrogradely
labeled neurons in the contralateral Gld mirrored the results seen on the ipsilateral side (Fig. 2E).
Retrogradely
labeled neurons projecting upon Cluster N (visualised green) mainly originate within the DLL, with few additional connections from the LdOPT and SpRt.
Not exact matches
Cyclic adenosine monophosphate (cAMP)- dependent protein kinase,
labeled with fluorescein and rhodamine on the catalytic and regulatory subunits, respectively, was injected into Aplysia sensory
neurons either in culture or in intact cell clusters.
For this particular study, the research team created a small library consisting of different fly lines; in each line, a different set of specific
neurons was genetically
labeled and could be artificially activated, with each
neuron type secreting a different neuropeptide.
Fluorescently
labeled synaptic vesicles inside the axons of cultured
neurons were recorded with stimulated emission depletion (STED) microscopy in a 2.5 - micrometer by 1.8 - micrometer field of view.
Donato emphasized that this
labeling step was key to closely tracking development among a crowd of
neurons, in addition to manipulating their activity at later stages of life.
The technique allowed him to zero in on and
label populations of
neurons that develop at the time of
labeling, without any previous understanding about their identity beyond their birthdate.
«By harnessing the lineage tracing methods that are available in the fly, he was able to adapt them to
label the glia and motor
neurons that are born from the same stem cell in two different colors.»
It turned out that 80 % of the BrdU -
labeled cells were also tagged with the enolase, confirming that the newborn cells were in fact
neurons.
The group employed various viral tracing methods — infecting receptor - expressing
neurons with a virus strain and watching them spread as they
label infected cells with a fluorescent protein — to visualize the neural circuit downstream of the ESP1 receptor, as well as providing an image of nerve fibers belonging to specific
neurons in the brain and synapses relaying impulses from
neuron to
neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.
Furthermore, the team devised a technique to control the number of
neurons labeled —
labeling too many
neurons makes it impossible to distinguish individual ones — that allows researchers to visualize individual
neuron shapes and trace their connecting fibers through intact tissues using another technology the Gradinaru laboratory has helped develop, known as tissue clearing.
Using a technology called VAST, which can automatically
label individual
neurons, glia, and blood vessels different colors, as well as smaller structures such as dendrites and mitochondria, the researchers analyzed the contents of three cylindrical chunks of brain tissue, each no bigger than grains of salt.
To create the
labels, the scientists split a fluorescent molecule in half, one half for the talking
neuron and one half for the listening
neuron.
Using their own recently - developed genetic
labeling techniques for tracking these cells in early development in mice, Taniguchi and his team observed that, like most
neurons, the cells remodeled their axonal organization through development.
Together, the present study suggests that GRPR +
neurons constitute a long - sought
labeled line for itch sensation in the spinal cord.
Chang got a genetic foothold on this sensory system by
labeling GPCRs in mice and engineering them to produce channelrhodopsin, a molecule that allowed the scientists to use light to manipulate the cells» function and study the
neurons sorted by different GPCRs.
The chemical
labels newly divided cells, and in their brain tissue, it showed up in a sprinkling of
neurons in the hippocampus — a seahorse - shaped structure involved in memory and learning.
A thalamocortical, or TC
neuron labeled with fluorescent dye, as used in Dr. Augustinaite's study.
Labeled antibodies, which bind to specific cell types, allowed the researchers to determine which types of
neuron were being created at which times.
To see if Narp played a role in making these new cells in mice, the researchers injected a synthetic molecule, BrdU, to
label and allow detection of newly created cells that would become
neurons in the hippocampus.
Before ending the experiment, the mice were injected with a compound that's selectively incorporated into dividing cells, and hence
labels adult - born
neurons.
In the model, the team used the technique to identify,
label, and manipulate a population of
neurons in the motor cortex that fired when a mouse pushed a lever to receive a reward in response to a stimulus.
Once the
neurons of interest were identified and
labeled, his team presented the stimulus to the mouse while optogenetically inhibiting the group of
neurons.
The study came about when, a few years ago, Cho and Kim were selectively
labeling and stimulating hippocampal
neurons that project to the mPFC, and examining how this manipulation affects fear memory formation in mice.
Thus, though we simultaneously recorded an average of four
neurons at a time per electrode penetration, we grouped all nonsimultaneously recorded data together, and discarded the trial
labels.
Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual
neurons in the brain, to
label cells stochastically using gene cassettes that code for four uniquely expressed fluorescent proteins.
At 13 months of age, the intracellular MOAB - 2 immunolabel was hardly detectable in pyramidal
neurons adjacent to amyloid plaques, the latter which were strongly
labeled by this antibody and not by pab27576 (Additional file3: Figure S2 a-b).
Human induced pluripotent stem cell
neurons imaged in phase contrast (gray pixels, left)-- currently processed manually with fluorescent
labels (color pixels) to make them visible.
A cortical layer 2/3
neuron at 21 days in vitro
labeled by ex utero electroporation with fluorescent marker for spine visualization.
In this study, we employed a method called optical pulse -
labeling, or OPL, which allowed us to see how the mutant huntingtin ravaged the brain over time —
neuron by
neuron.»
Synaptic reconstruction of all excitatory inputs made onto a single cortical pyramidal
neuron labeled with sparse in utero electroporation (Unpublished, Dan Iascone)
Synaptic reconstruction of all excitatory and inhibitory inputs made onto a single cortical pyramidal
neuron labeled with sparse in utero electroporation.
University of Calgary scientists say they think their research is the first to show that bisphenol - S, an ingredient in many products bearing «BPA - free»
labels, causes abnormal growth surges of
neurons in an animal embryo.
To achieve sparse
labeling of individual
neurons one could reduce the plasmids amounts to reduce transfection efficiency.
Both 3DISCO and CLARITY have relied on the use of transgenic mice in which a subpopulation of
neurons are brightly
labeled with GFP (e.g. Thy1 - YFP - H mice).
By
labeling HAR1 molecules in human and macaque embryos, we discovered that the RNAs functioned in
neurons during patterning and layout of the cortex, 6 a brain structure that expanded greatly in size during human evolution.7 Exactly which genes HAR1 is regulating remains to be determined.
Immunocytochemistry / Immunofluorescence of mixed
neuron - glial cultures
labelling rabbit GFAP (red channel) and chicken vimentin CPCA - Vim (green channel).
3D time - lapse of
neuron: Time - lapse image of mouse primary
neuron labeled with EGFP after co-culture with astrocyte for 2 weeks.
Triple -
labelled preparations in which mDA
neurons were identified by TH staining revealed that BMPR2 was expressed on, but not limited to, mDA
neurons in cultures of the E14 rat VM (Figure 1A, B).