Sentences with phrase «retroviral dna»
In a paper published in August in Science, scientists addressed this potential biohazard by knocking out the retroviral DNA with the gene editing technology CRISPR - Cas9.
http://discovermagazine.com/
«The integration of retroviral DNA is a relatively uncommon event compared with the number of viral DNA copies found in infected cells.
The team is now exploring just how widespread this retroviral DNA is in the modern human population and whether the viruses themselves are ever active.
Not exact matches
These retroviral gene sequences make up about 8 per cent of the human genome, and are part of what is called non-coding DNA because they don't contain genetic instructions to make proteins.
With the help of my colleagues I started growing retroviral vector - containing plasmids in bacterial cultures, isolating the plasmid DNA and transfecting special producer cell lines with these plasmids.
Analysis of retroviral recombinants formed after a single round of replication revealed that (i) the nonselected markers changed more frequently than expected from the rate of recombination of selected markers; (ii) the transfer of the initially synthesized minus strand strong stop DNA was either intramolecular or intermolecular; (iii) the transfer of the first synthesized plus strand strong stop DNA was always intramolecular; and (iv) there was a strong correlation between the type of transfer of the minus strand strong stop DNA and the number of template switches observed.
These data suggest that retroviral recombination is ordered and occurs during the synthesis of both minus and plus strand DNA.
Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS