Sentences with phrase «retroviral packaging cells»

Not exact matches

The cDNA library was packaged into retroviral particles and introduced into the J - Lat 6.3 cell line via infection (Table 1).

HEK293T cells were transfected with the following retroviral or lentiviral constructs, with their packaging vectors indicated in the parentheses, using FuGENE 6 reagent (Promega): pMX - puro - STAT5A * (provided by T. Kitamura, University of Tokyo; pCMV - Gag - Pol and pCMV - VSV - G); pLenti - EF - FH - TAZ - S89A (psPAX2 and pVSV - G); PLKO - NS and shTAZ lentiviral construct (provided by C. Linardic, Duke University; pCMV - Rev, pCVM - VSVG; pMDL).