Sentences with phrase «retroviral transfer»

«The ethical issue of retroviral transfer from pig to man (which is in theory facilitated from organs of the double knockout pigs) remains a major one.

In this study, we develop a reproducible protocol for efficient reprogramming mouse neural progenitor cells (NPCs) on human foreskin fibroblast (HFF) cells via retroviral transfer of human transcriptional factors OCT4 / SOX2 / KLF4 / C - MYC.

Not exact matches

This group is well known for its design of retroviral vectors for efficient gene transfer and it seemed like a good idea for me to go there for a year and exchange knowledge and expertise.

Analysis of retroviral recombinants formed after a single round of replication revealed that (i) the nonselected markers changed more frequently than expected from the rate of recombination of selected markers; (ii) the transfer of the initially synthesized minus strand strong stop DNA was either intramolecular or intermolecular; (iii) the transfer of the first synthesized plus strand strong stop DNA was always intramolecular; and (iv) there was a strong correlation between the type of transfer of the minus strand strong stop DNA and the number of template switches observed.

Molecular and cell biology of retrovirus - host cell interactions — Development and application of retroviral gene transfer and drug screening systems

Having been excited by the potential of retroviral gene transfer as a therapeutic, it has been a fascinating opportunity to get to grips with the various processes in the lab; particularly more recently when I have spent time working independently to optimise the process in various cell subsets.