Sentences with phrase «reverse phase hplc»
Adam used a wide range of techniques revolving around construction of His - Tag plasmids and eventual protein purification via Ni - NTA affinity and reverse phase HPLC.
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Monitoring oxidative stability and phenolic compounds composition of myrtle - enriched extra virgin olive during heating treatment by flame, oven and microwave using reversed phase dispersive liquid — liquid microextraction (RP - DLLME)- HPLC - DAD - FLD method.
The versatility of HPLC in compound separation is due mainly to the availability of numerous chromatographic matrices (reversed - phase, normal phase, ion - exchange, ion - pair, and others), its reproducibility (peak performance and results), and its selectivity (compounds of specific moieties / structures).
hIAPP containing the appropriate posttranslational modification (disulfide bridge and C - terminal amidation) was synthesized using solid - phase N - tert - butyloxycarbonyl chemistry at the W. Keck Facility at Yale University and was purified by reverse - phase HPLC.
Reversed - phase high - performance liquid chromatography (HPLC) with electrochemical detection was used to analyse ascorbic acid, based on the method of Iriyama et al. with modifications [29], [30].
Cells were then washed in medium, and metabolites were extracted with acetone and analyzed by reverse - phase high - performance liquid chromatography (HPLC) and by electrospray mass spectrometry as described previously (19).