Sentences with word «rhodamine»

A model is showing the charge transfer (e --RRB- mechanism of Rhodamine B molecules (top) interacting with N - doped graphene (bottom sheet) when excited with different laser lines, which leads to ultrasensitive molecular sensor with N doped graphene.

If nuclear counterstaining is desired, four - color antibody staining is possible using BV421, BV480, Alexa Fluor 488, and Rhodamine Red - X.

Cells were then fixed with two percent paraformaldehyde and stained with rhodamine phalloidin to allow visualization of filamentous actin.

Cyclic adenosine monophosphate (cAMP)- dependent protein kinase, labeled with fluorescein and rhodamine on the catalytic and regulatory subunits, respectively, was injected into Aplysia sensory neurons either in culture or in intact cell clusters.

When exposed to the red, fluorescent dye rhodamine B little dye accumulates in the cells and tissues of the fish embryos because Abcb4 prevents uptake of the dye as can be seen in the image above.

The fluorescence quantum yield values (Φf) of the fluorescent protein were calculated from integrated, blank, and spectrally corrected emission spectra relative to the standard Rhodamine 101 solved in ethanol (Φf = 0.915)[29] using the following formula [30].

Swapping out specific chemical building blocks in fluorescent molecules called rhodamines can...

Effective five - color fluorescent labeling is possible when BV421 and BV480 are combined with Alexa Fluor 488, Rhodamine Red - X, and Alexa Fluor 647 conjugates.

The actin cytoskeleton is stained with rhodamine - phalloidin.

Germinated seeds were dyed in 0.5 % rhodamine B for about two days, producing deeply dyed and strongly fluorescent cotyledons and embryos (Fig 1A, 1B and 1C).

It uses a rhodamine - based dye to absorb sunlight, which excites electrons in the dye and gives them enough energy to hop around.

In 2013, the researchers developed a fluorescent molecule called silicon - rhodamine (SiR), which switches «on» only when it binds to the charged surface of a protein like the ones found on the cytoskeleton.

Immunofluorescent analysis of HepG2 cells using 60008 -1-Ig (beta actin Antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

Immunofluorescent analysis of MCF - 7 cells, using OPTN antibody 10837 -1-AP at 1:25 dilution and Rhodamine - labeled goat anti-rabbit IgG (red).

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004 -1-Ig (GAPDH antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

For double labeling, the cells were incubated with primary antibodies against TuJ1 and L1 or GFAP, then biotinylated anti-mouse or rabbit (rat) IgG (Vector, Burlingame, CA, USA), and finally with avidin - rhodamine (Vector) and corresponding FITC - conjugated secondary antibodies (from different host species, Chemicon and Molecular Probes, Invitrogen).

Cells were washed (3 × 5 min) with 1 % BSA in PBS and probed with the secondary antibodies, rhodamine red - conjugated donkey anti-mouse IgM at 1:400 (Jackson ImmunoResearch Laboratories, West Grove, PA) and Alexa Fluor 488 donkey anti-goat IgG at 1:800 (Molecular Probes, Carlsbad, CA), for 1 h in the dark at RT..

Immunofluorescent analysis of HepG2 cells, using SMN2 antibody 11708 -1-AP at 1:25 dilution and Rhodamine - labeled goat anti-rabbit IgG (red).

Immunofluorescent analysis of Hela cells, using SMN2 antibody 11708 -1-AP at 1:25 dilution and Rhodamine - labeled goat anti-rabbit IgG (red).

Rhodamine 101 in ethanol was used as fluorescence quantum yield standard for both excitation conditions.

Cell samples (2.5 × 105 cells / mL) were treated with rhodamine - 123 (1.3 μmol / L; Sigma - Aldrich) in the presence or absence of cyclosporin A (5 μmol / L; Sigma - Aldrich) for 90 min at 37 °C.