«We were able to introduce a range of mutations at two positions within
the rhodopsin protein from Krokinobacter eikastus, which are known to be important for its pump activity,» lead author Masae Konno, from the Department of Frontier Materials at NITech, explains.
She wanted to connect chemistry to biology in some concrete way, so she'd planned to join a biochemistry lab focused synthesizing retinal (vitamin A) as a way to understand
rhodopsin proteins.
Not exact matches
The two receptors are homologous with each other,
rhodopsin, and other receptors coupled to guanine nucleotide regulatory
proteins and they contain seven hydrophobic domains, which may represent transmembrane spanning segments.
The rod outer segment is made up of stacked discs each containing many types of
proteins, in particular
rhodopsin, a light - sensitive pigment.
Using this material, the researchers were able to analyse in detail the response of
rhodopsin, the pigment
protein underlying vision, and phosphodiesterase, and for this reason they believe it can be useful in the field of optogenetics in the future.
When this clone was inserted into a mammalian expression vector and transfected into COS - 7 cells, a
protein that specifically phosphorylated the agonist - occupied form of the beta 2 - adrenergic receptor and phosphorylated, much more weakly, the light - bleached form of
rhodopsin was expressed.
Currently, we use Rosetta in conjunction with EPR distance and mobility measurements in order to model the
rhodopsin / G -
protein complex and conformational changes occurring as the G -
protein binds to
rhodopsin.
Rhodopsin is a G -
protein - coupled receptor (GPCR), which are integral membrane
proteins with seven transmembrane helices.
C - X-C chemokine receptor type 4 (CXCR4), also known as fusin or cluster of differentiation 184 (CD184), is a seven transmembrane (TM) G -
protein coupled receptor (GPCR) belonging to Class I GPCR or
rhodopsin - like GPCR family [1, 2].
Crystal structures exist for the G -
protein, as well as activated
rhodopsin bound to the C - terminal tail of Galpha, but not for the
rhodopsin / G -
protein complex.
RosettaTMH, with and without experimental restraints, performs better than the published MembraneAbinitio folding method and is capable of achieving native - like
protein topologies in most cases, including
rhodopsin.
Purifying membrane
protein and growing membrane
protein microcrystals of bacterial
rhodopsins and terminal oxidases.
He has also had a long - standing interest in targeting, translocation and integration of polytopic membrane
proteins including
rhodopsin and sodium - calcium exchangers.
One particular focus in our work is structure, dynamics, and interactions of G -
Protein Coupled Receptors (Wu, H.; et al. «Structure of a class C GPCR metabotropic glutamate receptor 1 bound to an allosteric modulator»; Science; 2014; 58 or Alexander, N.; et al. «Energetic analysis of the rhodopsin - G - protein complex links the alpha5 helix to GDP release»; Nat Struct Mol Biol; 201
Protein Coupled Receptors (Wu, H.; et al. «Structure of a class C GPCR metabotropic glutamate receptor 1 bound to an allosteric modulator»; Science; 2014; 58 or Alexander, N.; et al. «Energetic analysis of the
rhodopsin - G -
protein complex links the alpha5 helix to GDP release»; Nat Struct Mol Biol; 201
protein complex links the alpha5 helix to GDP release»; Nat Struct Mol Biol; 2014; 56).
To further examine the morphology of cells and the localization of
protein expression within the retina, immunohistochemical staining of both paraffin and OCT retinal sections was performed with the following antibodies (Table S1): human cone arrestin (for cone photoreceptors),
rhodopsin (for rod photoreceptors), RPE65 (for the retinal pigment epithelium, RPE), glial fibrillary acidic
protein (GFAP, for astrocytes and Müller cells), glutamine synthetase (for Müller cells) and G0alpha (for ON bipolar cells).