Sentences with word «ribosomal»

Sequencing of 16S ribosomal RNA genes revealed a relative abundance of Bacteroidetes and Verrucomicrobia bacteria in mice fed alcohol compared with a relative predominance of Firmicutes bacteria in control mice.

Biological Function of Ribosomal Protein L10 on Cell Behavior in Human Epithelial Ovarian Cancer Jimin Shi, Lingyun Zhang, Daibing Zhou, Jinguo Zhang, Qunbo Lin, Wencai Guan, Jihong Zhang, Weimin Ren, Guoxiong Xu J. Cancer 2018; 9 (4): 745 - 756.

This piece of DNA, called the 16S subunit of ribosomal RNA, does not change quickly over time, however, so it gives a very coarse timeline for the evolution of bacteria.

Phylogenetic analysis of the non-pathogenic genus Spiromastix (Onygenaceae) and related onygenalean taxa based on large subunit ribosomal DNA sequences.

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution.

After six to eight weeks, the microbial content of the mice's feces were analyzed by 16S ribosomal RNA gene sequencing, which showed that approximately 20 kinds of oral bacteria were present in these mice.

Mutations in TFIIH causing trichothiodystrophy are responsible for defects in ribosomal RNA production and processing.

The mechanism of action for translation enhancing elements is still obscure, particularly in the case of internal ribosomal initiation sites.

Cryo — electron microscopy of the ribosome - Sec61 complex and a three - dimensional reconstruction showed that the Sec61 oligomer is attached to the large ribosomal subunit by a single connection.

A deeply conserved, noncanonical miRNA hosted by ribosomal DNA.

The nascent chain was extended within the exit tunnel, making contacts with ribosomal components at distinct sites.

Researchers use a small piece of the gene for ribosomal RNA, or rRNA — a gene that is distinct in every species — to grab all the rRNA genes that are present in a liter of seawater.

The next step was to determine which of these sequences could function as internal ribosomal initiation sites.

Genes encoding ribosomal proteins, DNA replication and repair machinery, oxidative stress, and purine biosynthesis were differentially expressed in Wolbachia during the development of B. malayi female worms, suggesting a role of the bacteria in worm reproduction.

The study, conducted in both human and mouse cells, shows that cancer genomes lose copies of repetitive sequences known as ribosomal DNA.

Further, at a low cytostatic concentration (2 nM), ActD causes ribosomal stress leading to a decrease in HDM2 activity and thereby p53 stabilization and activation.

If all ribosomes were alike, people with mutations in ribosomal components should have malfunctions all over their bodies, or might not ever be born.

Tissue DNA was subjected to amplification of a region within the 5s - 23s ribosomal genes using a nested set of primers.

NOP2 and PPRC1 are involved in ribosomal biogenesis and are coexpressed with DKC1, a gene involved in ribosome biogenesis and also a component of the telomerase ribonucleoprotein enzyme complex.

This reporter employs a tandem array of internal ribosomal entry sites to drive translation of an enhanced Yellow Fluorescent Protein (Venus) from the transcript that normally encodes for the early endodermal marker Hex.

These studies only looked at a single copy part of individuals» genomes and never at ribosomal DNA.

The ribosome is made of RNA called ribosomal RNA (rRNA).

There are parallels to steps in the formation of a much more complex ribonucleoprotein, the 30S ribosomal subunit.

Coupling of Tetrahymena ribosomal RNA splicing to beta - galactosidase expression in Escherichia coli

«One striking example is the link between ribosomal protein Rpl26 and body weight,» says Hao Li, first author of the paper.

Following a lead from these experiments, we obtained direct evidence for differential stoichiometry among core ribosomal proteins in unperturbed wild - type cells.

Relative quantification of original input RNA amounts were calculated by comparison to standard curves using purified PCR product as a template for the mRNAs of interest and were normalized to the amount of acidic ribosomal phosphoprotein P0 mRNA [24,46].

Data were normalized to acidic ribosomal phosphoprotein P0 mRNA levels [24,46].

From the unique genes during night - time three biological processes were found to be associated with ribosomal metabolism, peptide synthesis / modification / processing, and ATP synthesis and oxidative phosphorylation.

The Chr 16 segment contains about two thirds of the human Chr 21 homologues in the mouse, from mitochondrial ribosomal protein L39 (Mrpl39) gene to the distal telomere.

If you haven't included ribosomal RNA in you mapping reference and are working on mouse, a red flag for rRNA contamination is particularly high expression of genes called CT010467.1, AY036118, Erf1, or Gm42418.

I thought there must be some sequences that have been retained during evolution, and that started me on the long path of studying eukaryotic ribosomal RNA using evolution as a guide.

At low cytostatic concentrations, ActD causes ribosomal stress, which decreases MDM2 activity, resulting in p53 stabilization and activation.

The phylogenetic position of Rhopalura ophiocomae (Orthonectida) based on 18S ribosomal DNA sequence analysis.

The chemical mechanisms of RNA - catalyzed reactions are discussed with emphasis on the self - splicing ribosomal RNA precursor of Tetrahymena and the enzymatic activities of its intervening sequence RNA.

In vivo mutational analysis of this nucleotide indicates that it has an essential role in ribosomal function.

The force - sensing peptide VemP employs extreme compaction and secondary structure formation to induce ribosomal stalling.

Today, Ban's group of 15 to 20 scientists still works on ribosomes, using a combination of crystallography, electron microscopy, and biochemical experiments to focus on what happens to newly synthesized proteins as they get folded and released by ribosomal complexes.

That was surprising, he explains, because «fibrillarin resides deep within the nucleolus of the host cell... [where it] methylates ribosomal RNA molecules, which then go on to form ribosomes,» but its full function may not be completely understood.

Ribosomes, the cellular factories that manufacture proteins, contain both RNA and protein, but exactly how all of the different ribosomal components contribute to protein synthesis is still not clear.

«Ataluren stimulates ribosomal selection of near - cognate tRNAs to promote nonsense suppression.»

We examined 13,355 prokaryotic ribosomal RNA gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut microbial diversity.

This set includes various ribosome builders, as well as other proteins that process and transcribe ribosomal RNA, a necessary step before the ribosomes can be assembled and pushed out of the nucleolus.

Both substrate analogs are contacted exclusively by conserved ribosomal RNA (rRNA) residues from domain V of 23S rRNA; there are no protein side - chain atoms closer than about 18 angstroms to the peptide bond being synthesized.

In particular, there was a complex between the 40S ribosomal subunit and initiator tRNA that went away when heme was missing or double - stranded RNA was present.

«We have ample evidence that hundreds of the oldest ribosomal proteins still start with a valine or a leucine code and do not have the codon for methionine in the DNA,» Duax said, referring to proteins found in basic cell components called ribosomes.

Even with the advent of DNA sequencing and genetic identification, this was generally done with one particular ribosomal gene.

The interaction of EF - G with ribosomal elements implicated in stimulating catalysis, such as the L10 - L12 stalk and the L11 region, and of domain IV of EF - G with the tRNA at the peptidyl - tRNA binding site (P site) and with mRNA shed light on the role of these elements in EF - G function.