Evidence from 18S
ribosomal DNA that the lophophorates are protostome animals.
Phylogenetic analysis of the non-pathogenic genus Spiromastix (Onygenaceae) and related onygenalean taxa based on large subunit
ribosomal DNA sequences.
The organism was identified as Nocardia abscessus (formerly Nocardia asteroides type 1) based on 16S
ribosomal DNA sequencing of samples extracted from cultures and unstained cytologic smears.
In addition, the monopolin complex maintains the integrity and silencing of
ribosomal DNA (rDNA) repeats in the nucleolus.
Homology between
the ribosomal DNA of Escherichia coli and mitochondrial DNA preparations of maize is principally to sequences other than mitochondrial rRNA genes
A deeply conserved, noncanonical miRNA hosted by
ribosomal DNA.
Partial copies of the extrachromosomal
ribosomal DNA (rDNA) element are found at the ends of each chromosome, suggesting a novel telomere structure and the use of a common mechanism to maintain both the rDNA and chromosomal termini.
For three, he saw a loss of
ribosomal DNA copies in the cancer cells relative to the normal cells.
The study, conducted in both human and mouse cells, shows that cancer genomes lose copies of repetitive sequences known as
ribosomal DNA.
First author Baoshan Xu, Ph.D., a former postdoctoral research associate in the Gerton Lab now starting his own lab at Sun Yat - sen University in China, worked with Hua Li, Ph.D., to use computational methods to count the number of copies of
ribosomal DNA in normal and cancer cells of 162 patients from the eight projects.
Previous studies on budding yeast showed that whittling down the number of copies of
ribosomal DNA created a genome that was very sensitive to DNA damage.
The cells somehow managed to be highly proliferative, made more ribosomal RNA, and synthesized more protein, all with fewer copies of
ribosomal DNA.
This time, Xu used a sophisticated technique called droplet digital PCR to count
the ribosomal DNA copies in normal and cancer cells from the mice.
The few studies that have included analysis of
ribosomal DNA thus far have shown that the number of copies of the repetitive sequences expand and contract all the time.
These studies only looked at a single copy part of individuals» genomes and never at
ribosomal DNA.
The study, published in Science and co-authored by University of Cambridge and King's College London, shows that the genetic variation of
ribosomal DNA (rDNA) could be driving how the environment within the womb determines an offspring's attributes.
Lead researcher Professor Vardhman Rakyan from QMUL said: «The fact that genetic variation of
ribosomal DNA seems to play such a major role suggests that many human genetics studies could be missing a key part of the puzzle.
We analyzed ∼ 78 million base pairs of unique DNA sequence and 2062 polymerase chain reaction — amplified 16S
ribosomal DNA sequences obtained from the fecal DNAs of two healthy adults.
Not exact matches
Genes encoding
ribosomal proteins,
DNA replication and repair machinery, oxidative stress, and purine biosynthesis were differentially expressed in Wolbachia during the development of B. malayi female worms, suggesting a role of the bacteria in worm reproduction.
This piece of
DNA, called the 16S subunit of
ribosomal RNA, does not change quickly over time, however, so it gives a very coarse timeline for the evolution of bacteria.
«We have ample evidence that hundreds of the oldest
ribosomal proteins still start with a valine or a leucine code and do not have the codon for methionine in the
DNA,» Duax said, referring to proteins found in basic cell components called ribosomes.
Even with the advent of
DNA sequencing and genetic identification, this was generally done with one particular
ribosomal gene.
Sogin began collecting and sifting through marine organisms — algae, fungi, sponges, jellyfish, anemones, mollusks — cutting them up and extracting
DNA, adding enzymes, concentrating the
DNA and sequencing the genes, reducing them to strips of code, comparing their
ribosomal RNA, and applying algorithms to measure their relationship with one another and with insects, worms, fish, birds, and mammals.
Following the isolation and purification of microbial
DNA from all samples, we used community fingerprinting (ARISA) and high - throughput sequencing of the 16s
ribosomal RNA gene (V1 - V3) to describe the microbial community structure in each group.
DNA repair efficiency in transgenic mice over expressing
ribosomal protein S3.
The remaining genes provide instructions for making molecules called transfer RNAs (tRNAs) and
ribosomal RNAs (rRNAs), which are chemical cousins of
DNA.
We partitioned the alignment into
ribosomal RNA; tRNA; a control region; and the first, second, and third codon positions for coding
DNA sequences (CDSs) and followed previously described procedures (9) to perform Bayesian analyses in MrBayes (73) and Beast (74).
The 50 Mb houses no more than 25 essential loci and consists largely of satellite
DNA sequences,
ribosomal genes, and transposable elements (John and Miklos, 1988).
Tissue
DNA was subjected to amplification of a region within the 5s - 23s
ribosomal genes using a nested set of primers.
BAZ2A is an essential component of the nucleolar remodeling complex (NoRC), which mediates recruitment of histone modifyine enzymes and
DNA methylase involved in the silencing of
ribosomal RNA transcription by RNA polymerase I. BAZ2B is believed to be involved in regulating nucleosome mobilization along linear
DNA.
Unlike typical eukaryotic organisms, Tetrahymena has two nuclei — a micronucleus that contains normal chromosomes and a macronucleus whose chromosomes are fragmented into thousands of small pieces of
DNA that all encode the same
ribosomal RNA gene.