Sentences with phrase «ribosome binding»

To overcome this challenge, the students altered a sub-unit of curli DNA, called ribosome binding sites (RBS), and then used these modified RBS to specifically tune protein production, explained Erin Kim, A.B.» 19, a molecular and cellular biology concentrator.

Among the key elements engineers need to get an organism such as E. coli to make a protein from a synthesized gene are extra sequences such as promoters (to help the cell make RNA from DNA) and ribosome binding sites (or RBS, which the cell needs to make proteins from the RNA).

Crystal structures of the ribosome bound to elongation factors provide insights into translocation and decoding.

The paper solves the structure of a bacterial ribosome bound to a messenger RNA containing a loop that regulates translation.

Not exact matches

At the next stage, RNA molecules began to synthesize proteins, first by developing RNA adaptor molecules that can bind activated amino acids and then by arranging them according to an RNA template using other RNA molecules such as the RNA core of the ribosome.

«A new class of antibiotics to combat drug resistance: Newly discovered antibiotic binds to ribosome, disrupts protein synthesis.»

Here we report the crystal structure of EF - P bound to the Thermus thermophilus 70S ribosome along with the initiator transfer RNA N - formyl - methionyl - tRNAi (fMet - tRNAifMet) and a short piece of messenger RNA (mRNA) at a resolution of 3.5 angstroms.

Elongation factor P binds to the ribosome so as to position the initiator transfer RNA for the first bond formation.

To begin to answer that question, the researchers modeled different combinations of promoters and ribosome - binding sites, finding that they could predict about 70 percent of the variance in the combinations.

The pilot project tested a dozen or so of the most commonly used gene promoters (regions of DNA that facilitate gene transcription) and segments of DNA that encode ribosome - binding sites (sequences of messenger RNA that control protein translation) to determine whether they behave consistently in different cellular contexts.

«The bactericidal mechanism of ODLs and the fact that they bind to a site on the ribosome not exploited by any known antibiotic are very strong indicators that ODLs have the potential to treat infections that are unresponsive to other antibiotics,» said Mankin, who is also professor of medicinal chemistry and pharmacognosy.

The UIC researchers, including graduate student Tanja Florin and postdoctoral research associate Malgorzata Dobosz - Bartoszek, also found that when bound to the ribosome, the antibiotic disrupts its ability to interpret and translate genetic code.

Riboswitches — so named because they meddle with the ribosome — are portions of specific mRNAs that bind to a metabolite.

When the drug is present, it binds within the tunnel of the ribosome and interacts with the translating leader peptide to stall protein synthesis.

Short strands of RNA called siRNA bind to the messenger RNA that carries this genetic information, preventing it from reaching the ribosome.

A purified yeast Sec61 complex bound to monomeric yeast ribosomes as an oligomer in a saturable fashion.

Kiebler and his group had previously shown that the brain - specific RNA - binding protein Staufen2 (Stau2) plays a central role in the transport of mRNAs from the cell nucleus to synaptic ribosomes.

In normal cap - dependent translation, the ribosome is recruited to the 5» end of mRNA via a specialized cap - binding complex.

For this study, researchers used an approach called structure - based design to re-engineer how spectinomycin binds to the ribosome.

To do that, the spectinamides bind to a particular site on ribosomes that is not shared by other TB drugs.

To guide their design efforts, scientists used a 3 - D model that provided an atomic - level view of spectinomycin bound to the tuberculosis ribosome.

This unusual shape prevents ribosomes — the button - shaped organelle that zips along the messenger RNA and strings together amino acids into proteins — from binding to the appropriate piece of RNA.

The rescue proteins recognize arrested ribosomes by detecting, and binding to, conserved locations normally occupied by mRNA.

To visualize translation, Dr. Singer and his colleagues took advantage of a key occurrence during the first round of translation: the ribosome to which mRNAs attach must displace so - called RNA - binding proteins from the mRNAs.

As a result, these mRNA molecules — stripped of their green fluorescent proteins, bound to ribosomes, and ready to be translated into a protein — appear red.

While on viable mRNAs UPF1 is removed by the protein factories, the ribosomes, it remains bound to defective mRNAs and recruits additional enzymes that cause the degradation of the mRNA «The protein UPF1 bound to the mRNA acts as an armed trap that only has to be triggered when needed to degrade the defective mRNA,» says Zünd.

Each of the twenty - one amino acids used by the human body has a tRNA binding partner that carries it to the ribosome where it can be incorporated at the appropriate point into a growing protein.

A protein domain that is found in many RNA - binding proteins that are required for mRNA synthesis, pre-mRNA splicing, ribosome biogenesis and RNA decay.

hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)- mediated translation.

A Ribosome Assembly Factor Ebp2p, the Yeast Homolog of EBNA1 - Binding Protein 2, Is Involved in the Secretory Response.

Wolin and her colleagues had begun to answer questions about Ro's function when their earlier experiments demonstrated that the Ro protein binds to small RNA molecules that are misfolded versions of those that help make up the cell's ribosomes, which are its protein assembly lines.

Once bound to amino acids, the tRNAs (also called amino - acyl tRNAs) will seek out ribosomes.

The ribosome has two parts, which bind on either side of the mRNA.