Not exact matches
To that end, in collaboration with the University of Zurich and MD Anderson Cancer Center, the researchers tested melanoma
tumor samples from human patients undergoing treatment with the
same targeted therapies.
Thirty companies and academic groups will receive the
same set of
tumor samples from individual patients and will try to predict the best neoantigens.
Searching for T cells that had infiltrated the
tumors, they collected as many as 50 T cell
samples from a single
tumor; next, the team tested each batch of T cells against other
tumor samples from the
same patient.
There is considerable interest in finding CNAs that affect the
same chromosomal region in multiple
tumor samples.
Tumor analyses commonly employ a correction with a matched normal (MN), a
sample from healthy tissue of the
same individual, in order to distinguish germline mutations from somatic mutations.
Plasma IL - 6 and
tumor necrosis factor α (TNF - α) levels and chamber - fluid IL - 6, TNF - α, and IL - 1β levels were assayed using Quantikine High Sensitivity Immunoassay kits (R&D Systems, Minneapolis, Minn), per kit instructions, as described elsewhere.47
Samples were run undiluted in duplicate, and all samples for a couple were run at the sam
Samples were run undiluted in duplicate, and all
samples for a couple were run at the sam
samples for a couple were run at the
same time.