Sentences with phrase «satellite cell»

* Duration of a few weeks * An average dose more equivocal to a «loading» phase than a «maintenance phase» * A larger dose for potential responders who lack natural, dietary creatine * A smaller dose for potential non-responders with a significant amount of existing dietary creatine intake * A training protocol that emphasizes all phases of muscular energetics to take advantage of the ATP - CP, glycolytic, and oxidative effects of creatine supplementation (HIIT is ideal for cardiovascular exercise when supplementing creatine, due to the repeated bouts of high intensity work) * A training protocol that incorporates negatives in order to stimulate satellite cell fusion, as per Dr. Hatfield's theory of holistic training * A training protocol that emphasizes repeated bouts of work per the results of creatine studies * A nutrition protocol tailored to reduce post-workout cortisol levels, which would involve a post-workout shake and possible glutamine supplementation * A nutrition protocol that takes advantage of carb - load (super compensation) near the end of the cycle

The liberation of IL - 4 and IL - 6 stimulates satellite cell proliferation and fusion.

Genetic factors such as myostatin levels and satellite cell density play a large role in how much muscle an athlete can expect to gain.

A possible long - term benefit to muscle growth associated with whey protein, increased satellite cell recruitment, also has not been seen with soy protein.

Another positive effect is the satellite cell activity which makes your muscles able to expand continuously.

Potent myofiber hypertrophy during resistance training in humans is associated with satellite cell - mediated myonuclear addition: a cluster analysis.

Failure of satellite cell activation is also believed to cause skeletal muscle loss as these cells fuse with the muscle fibres and maintain their normal functioning.

He presented results from both SMA mouse models and patients that demonstrated SMN depletion results in muscle - intrinsic defects such as reduced satellite cell number, a delay in the myogenic program, reduced myoblast fusion, and molecular homeostasis disruption.

Studies of satellite cell activation via nitric oxide have opened an exciting area of research on muscle regeneration, growth and the muscle - fiber cytoskeleton that integrates mechanical and biochemical signal transduction with cell and molecular biology of muscle tissue in growth, development, disease, aging and evolution.

regulation of satellite cell activation and quiescence in different species (fish, mouse, human)

Topics such as the mechanisms of cell injury in normal and dystrophic muscle, compensatory muscle regeneration and hypertrophy, and the effects of various therapies or voluntary exercise on muscle repair, satellite cell activation, muscle growth, bone density and age - related atrophy are examined using a large variety of cellular, molecular and whole - animal in vivo assays of function.

Human satellite cell cultures were precultured for 4 days to different insulin concentrations, and the content of intracellular glucose and G6P was determined in the basal and insulin - stimulated state and glycogen was determined in the basal state in cultures as described in research design and methods.

Cells were subcultured twice (4 — 6 weeks of satellite cell proliferation) before final seeding in 100 - mm petri dishes.

Human satellite cell cultures display numerous features of mature skeletal muscles (1) and have been used for studies of muscle metabolism in cultures established from patients with type 2 diabetes and healthy control subjects (1 — 5,8,9,21,22).

Human satellite cell cultures were precultured for 4 days at different insulin conditions, and the glucose transport activity was determined under basal and after acute insulin stimulation as described under research design and methods.

The appearances under phase - contrast microscope of human satellite cell cultures during proliferation and differentiation are shown in Figs. 1a and b. Cell cultures were allowed to differentiate for 4 days before cell cultures were exposed to the different insulin concentrations for 4 days as described in research design and methods.

(14,15)-RRB- have found age - related satellite cell attrition consistent with the senescence of a subset thereof; moreover, one such study (15) reported that decreases in the number and quality of satellite cells with aging are reliably associated with elevated expression of p16Ink4a (contrary to (14)-RRB-, and with secretory and proteomic abnormalities consistent with a rising burden of senescent cells.

We determined the glycogen synthase (GS) activity; the content of glucose -6-phosphate, glucose, and glycogen; and the glucose transport in satellite cell cultures established from diabetic and control subjects.

Effect of preculturing human satellite cell cultures in increasing concentrations of insulin on the basal and insulin - stimulated glucose transport activity in cultures of human myofibers.

Recently, we described optimized conditions for satellite cell proliferation and differentiation (1).

Human satellite cell cultures were exposed for 4 days to different insulin conditions, and the GS activity was measured at 0.1 and 10 mmol / l G6P under basal and after acute insulin stimulation (Ins.

Effect of preculturing human satellite cell cultures in increasing concentrations of insulin on the activity constant A0.5 of GS.

The aim of the present study was to evaluate glucose transport and GS activity in human satellite cell cultures established from type 2 diabetic and control subjects.

The present study shows that the basal glucose uptake as well as insulin - stimulated GS activity is reduced in satellite cell cultures established from patients with type 2 diabetes.

Post-transcriptional regulation of satellite cell quiescence by TTP - mediated mRNA decay.

3D timelapse analysis of muscle satellite cell motility.

Concerning the first area, work from different laboratories has demonstrated that both environmental and cell - autonomous signals alter satellite cell regenerative potential with aging.

Apparently it's not until then that a satellite cell makes the effort to turn off all of its non-muscle options.»

But in disease conditions like muscular dystrophies, satellite cells can't keep up with repeated cycles of injury and are ultimately exhausted or functionally impaired,» Hindi said.

In a study in the current issue of the Journal of Neuromuscular Diseases, researchers treated these stem cells with leukemia inhibitory factor (LIF), which effectively maintained the undifferentiated state of the satellite cells and enhanced their transplantation efficiency.

When muscle injury occurs, a complex chain of signals prompts the satellite cells to awaken and generate new muscle cells to repair the injury.

«In normal conditions, skeletal muscle is a self - healing tissue and can recover promptly from most trauma because of the satellite cells.

Specialized stem cells known as satellite cells reside in skeletal muscle in an inactive state.

When Pax7 is missing or reduced, the satellite cells undergo premature differentiation, or lose their stem properties and their ability to regenerate injured muscles.

«These progenitor cells resemble adult muscle stem cells called «satellite cells» that can theoretically grow an entire muscle starting from a single cell.»

In a new study, researchers at Karolinska Institutet have investigated the number of mutations that accumulate in the muscle's stem cells (satellite cells).

From this tissue, Post isolated satellite cells, adult stem cells needed to replace dead muscle cells.

Lack of evidence for GDF11 as a rejuvenator of aged skeletal muscle satellite cells.

«This is a clean demonstration that the physiological and metabolic benefits of exercise radiate to skeletal muscle satellite cells, the adult stem cells responsible for repair after injury, even in senescent animals,» said Thoru Pederson, Ph..

«Our study found that by introducing an inhibitor of the STAT3 protein in repeated cycles, we could alternately replenish the pool of satellite cells and promote their differentiation into muscle fibers,» said Alessandra Sacco, Ph.D., assistant professor in the Development, Aging, and Regeneration Program at Sanford - Burnham.

Second, the pool of satellite cells needs to be replenished so there is a supply to repair muscle in case of future injuries.

First, when muscle is damaged by injury or degenerative disease such as muscular dystrophy, muscle stem cells — or satellite cells — need to differentiate into mature muscle cells to repair injured muscles.

They found that the inhibitor initially promoted satellite - cell replication, followed by differentiation of the satellite cells into muscle fibers.

In the late 1980s, Albertini's group began to focus on a group of satellite cells that surround the oocyte as it begins to grow and mature in the follicle.

But some signal provided by muscular injury or degeneration prompts satellite cells to start dividing and then to integrate themselves into damaged fibers, repairing the muscle tissue.

The researchers harvested satellite cells from both healthy and injured muscle tissue of young mice and from healthy tissue of old mice; extracted these cells» DNA with the histone coatings intact; and used tagged antibodies targeting the different kinds of marks to find which spots on those histones were flagged with either «stop» or «go» signals.

Rando's group is now looking to test whether the signatures they've identified in satellite cells generalize to other kinds of adult stem cells as well.

But when you look at these satellite cells the way we did, they seem ready to become all kinds of cells.

The differences between quiescent and activated cells, Rando's team found, are mirrored by those between young and old quiescent satellite cells.

Instead, they found, in quiescent satellite cells taken from the younger mice, copious instances in which histones in the vicinity of genes ordinarily reserved for other tissues were marked with both «stop» and «go» signals, just as genes associated with development to mature - muscle status were.