Each staple strand is made up of a specific sequence of bases (adenine, cytosine, thaline and guanine — the building blocks of DNA), which is designed to pair with specific subsequences on
the scaffold strand.
A clever strategy involving a series of bridges and loops was used to properly route
the scaffold strand, allowing it to pass through the entire structure, touching all lines of the wireframe once and only once.
Scaffolded DNA origami utilizes numerous chemically synthesized, short DNA strands (staple strands) to direct the folding of a larger, biologically derived strand of DNA (
scaffold strand).
The staple strands are introduced into a solution containing
the scaffold strand, and the solution is then heated and cooled.
The standard for DNA origami has long been limited to
a scaffold strand that is made up of 7,249 bases, creating structures that measure roughly 70 nanometers (nm) by 90 nm, though the shapes may vary.
«First we had to develop a custom
scaffold strand that contained 51 kilobases.
Not exact matches
In the high churches they saunter through the liturgy like Mohawks along a
strand of
scaffolding who have long since forgotten their danger.
Researchers match patches of the
scaffold to complementary
strands of DNA called staples, which latch on to their targets in two separate places.
It starts with a long DNA
strand — called a
scaffold — that has a precise sequence of the four molecular units, or nucleotides, dubbed A, C, G, and T, with which DNA spells out its genetic code.
Guided by the
scaffold pattern, the ensemble promptly organized itself into elongated muscle
strands, complete with onboard arteries and veins.
Researchers at McGill University have developed a new, low - cost method to build DNA nanotubes block by block — a breakthrough that could help pave the way for
scaffolds made from DNA
strands to be used in applications such as optical and electronic devices or smart drug - delivery systems.
The scientists also exercised the resulting muscle
strands in a bioreactor by affixing them to a soluble sugar
scaffold and slowly built tension using doughnut - shaped anchor points — essentially helping the muscle to «bulk up.»
The order of bases in each short
strand acts as a blueprint that dictates the
scaffold DNA's final, 3 - D shape.
BOND OF LIFE A link between nitrogen and sulfur atoms (bond shown in green) helps hold together protein
strands (yellow and white) that create a cellular
scaffold essential for tissue and organ formation.
The basic idea of DNA origami is to use a length of single -
stranded DNA as a
scaffold for the desired shape.
«We're using precisely shaped DNA constructs made as a
scaffold and single -
stranded DNA tethers as a programmable glue that matches up particles according to the pairing mechanism of the genetic code — A binds with T, G binds with C,» said Wenyan Liu of the CFN, the lead author on the paper.
Two research teams present two different methods for using single
strands of DNA to link various nanoparticles into complex 3D arrays: one using DNA hairpins for dynamic reconfiguration and the other using a DNA origami
scaffold.
We addressed this 2-fold problem by developing the following methods: (1) production of the largest to - date biologically derived single -
stranded scaffold using a λ / M13 hybrid virus to produce a 51 466 - nucleotide DNA in a circular, single -
stranded form and (2) inexpensive DNA synthesis via an inkjet - printing process on a chip embossed with functionalized micropillars made from cyclic olefin copolymer.
Boy is
stranded on
scaffold while man attempts to kill him.
They had to find the right blend of polymers to be both flexible and strong, which turned out to be a one with long - chain polymers that have
strands like spaghetti and one with shorter chain polymers that act like a
scaffold.