Sentences with phrase «score different antibodies»
One point of contention is whether validation should be considered «pass / fail,» or whether it should yield a ranking to score different antibodies as better or worse than others for the same target and application.
http://www.sciencemag.org/ Not exact matches
The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.