Sentences with phrase «secondary antibodies»

After washing, membranes were incubated at room temperature for 1 h in TBS / 0.05 % Tween 20 buffer with the IRDye800 secondary antibodies (1:10000) and then washed again in TBS / 0.05 % Tween 20 for 3 times.

Appropriately coupled secondary antibodies (Molecular Probes) were used for single and double labeling.

Awarded for consistently receiving multiple positive reviews on SelectScience ® for our Affinity - Purified Secondary Antibodies.

«Secondary antibodies from Jackson ImmunoResearch are easy to handle, effective, very reproducible (even through batches) and have very low background.

We manufacture the most comprehensive range of secondary antibodies and related products.

Primary antibodies were incubated overnight at 4 °C, washed with PBS and then incubated with the secondary antibodies at 37 °C for 1 hour.

Secondary labeling was carried out using isotype matched Alexa - fluor labeled secondary antibodies (AF488, 568 and 594, all from Invitrogen Corp).

HuProt Cross-Section: microarray probed with a serum Ig sample and fluorescently - labeled secondary antibodies to the Ig and common epitopes of the arrayed proteins.

The next day the cells were washed with PBS before fluorescent - conjugated secondary antibodies were added and incubated for an hour at room temperature.

The following primary antibodies were used: Oct - 4 (19857 Abcam) 1 ∶ 1000; β - III tubulin clone SDL.3 D10 (T8660 Sigma) 1 ∶ 500; Nestin (611658 BD Transduction laboratories) 1 ∶ 500 and TH (P40101 Pel - Freez) 1 ∶ 500, and as secondary antibodies: Alexa Fluor 594 Goat Anti-Mouse, Alexa Fluor 488 Goat Anti-Rabbit, Alexa Fluor 594 Goat Anti-Rabbit.

All secondary antibodies were tested for cross reactivity and non-specific binding.

Chicken IgY domains are especially useful for multi-color staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or - rabbit secondary antibodies.

Sections were blocked for 1 h at RT in blocking buffer (PBS supplemented with 1 % w / v BSA, 0.3 % Triton X-100, and 1 % v / v donkey serum), which was used to dilute primary and secondary antibodies in later steps.

Alexa Fluor 488 - and / or 594 - conjugated secondary antibodies (1:500; Invitrogen) reactive to the species of the primary antibodies, and the Olympus IX70 inverted microscope, were used for visualization.

Secondary antibodies were anti-rabbit Cy2, anti-mouse Cy3, and anti-rabbit-Cy5 at 1/300 (Jackson ImmunoResearch).

Following incubation for 60 min at 37 °C, the slides were washed extensively with PBS and secondary antibodies conjugated to fluorophores were added for 30 min.

It's best to select primary antibodies raised in different species, or at least from different IgG subclasses, so that secondary antibodies exclusively recognize only one primary antibody.

The membranes were incubated with primary antibodies against p - Smad1 / 5 (1:1000), Smad1 / 5 (1:1000; Abcam) or β - actin (1:2000) overnight at 4 °C, washed, incubated with the appropriate goat IR700 / 800 - labelled secondary antibodies (1:10000; LICOR), washed and visualized with Odyssey (LICOR).

For secondary antibodies, the sections were incubated with Alexa Fluor 546 - conjugated donkey anti-goat IgG antibody (Molecular Probes, Eugene, OR), Alexa Fluor 488 - conjugated donkey anti-rabbit IgG antibody (Molecular Probes) or Alexa Fluor 488 - conjugated donkey anti-mouse IgG antibody (Molecular Probes), Alexa Fluor 546 - conjugated goat anti-mouse IgG2b, or Alexa Fluor 488 - conjugated goat anti-mouse IgM, and then were counterstained with 1 µg / ml Hoechst 33258 for nuclear staining.

Secondary antibodies were horseradish peroxidase linked.

Sections were blocked for 1 h at RT in blocking buffer [PBS supplemented with 1 % w / v bovine serum albumin (BSA), 0.3 % Triton X-100, and 1 % donkey serum], which was also used to dilute primary and secondary antibodies in subsequent steps.

The sections were then washed and incubated in the appropriate biotinylated secondary antibodies (1:1000 Vector Laboratories) for 1 h, followed by a 1 h incubation with avidin - biotin complex (Vectastain Elite, Vector Laboratories).

They all typically require secondary antibodies, frequently of the mouse or rabbit variety.

Two secondary antibodies were incubated with the sections (Alexa Fluor #A21206, 488 donkey anti-rabbit IgG, 1:500 dilution; and Alexa Fluor #A10037, 568 donkey anti-mouse IgG, 1:500 dilution) for 2 h at RT and protected from light.

Primary antibodies specific to Oct4 and Nanog were detected using Alexa 568 conjugated secondary antibodies (red).

HRP - polymer secondary antibodies use micropolymer technology to form smaller detection complexes that allow better tissue penetration and sensitivity.

As long as you are not concerned that the anti-GST may interfere with your primary antibody, you may add both antibodies at the same time, and then perform simultaneously detection with the different secondary antibodies.

A diverse portfolio of high - quality primary and secondary antibodies is available for use in cancer research, epigenetics, immunology, neuroscience, and stem cell research as well as validated for multiple applications, including flow cytometry, western blotting, and immunoprecipitation.

Alexa - 488, Alexa - 594, and Alexa - 647 conjugated secondary antibodies (Invitrogen) were used to visualize primary antibody binding.

Rabbit anti-CCHa2 (1:1,000)[19], rabbit anti-Dilp2 (1:2,500; T. Ida), rabbit anti-Dilp2 (1:2,000, a gift from T. Nishimura, RIKEN Center for Developmental Biology)[27], rabbit anti-Dilp5 (1:2000, a gift from T. Nishimura)[27], mouse anti-Repo c8D12 (1:100; DSHB), mouse anti-GFP (1:500; Invitrogen), and rabbit anti-NPF (1:500; Ray Biotech, Inc) were used as primary antibodies, with anti-mouse-Alexa 488 (1:500; Invitrogen), anti-mouse-Cy3 (1:500; Jackson ImmunoResearch), anti-rabbit-Alexa 488 (1:500; Invitrogen), and anti-rabbit-Cy3 (1:500; Jackson ImmunoResearch) used as secondary antibodies.

Generally, the IHC kits include anti-IgG secondary antibodies.

Secondary antibodies conjugated with either AF680 (Invitrogen Corporation) or CW800 (LI - COR Biosciences, Lincoln, NE, USA) were used to visualize protein bands using the Odyssey Infrared Imager (LI - COR Biosciences).

Tip 6: For detection of immunoprecipitated proteins by Western blotting, without or reduced detection of non-specific artifacts (such as the heavy and light chains of the immunoprecipitating antibody), detect primary antibodies using HRP - conjugated anti-rabbit light chain - specific (L) antibody and HRP - conjugated Protein A instead of traditional HRP - conjugated secondary antibodies.

For double labeling, the cells were incubated with primary antibodies against TuJ1 and L1 or GFAP, then biotinylated anti-mouse or rabbit (rat) IgG (Vector, Burlingame, CA, USA), and finally with avidin - rhodamine (Vector) and corresponding FITC - conjugated secondary antibodies (from different host species, Chemicon and Molecular Probes, Invitrogen).

We manufacture and supply most standard inventory secondary antibodies in bulk volumes upon request.

Unfortunately, many commercial preparations of BSA, even some of the highest purity grades, contain IgG that may become an antigen for cross-reacting secondary antibodies.

Background may derive from sticky soluble immune complexes or from contaminating bovine IgG sticking non-specifically and attracting cross-reacting labeled secondary antibodies.

The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug / ml for 1hour at room temperature.

F (ab») 2 fragments are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors ot to Protein A or Protein G.

Generation, purification, and testing of primary antibodies Gold -, enzyme - and fluorescent labeling of secondary antibodies In situ hybridization Light - and electron microscopy

Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

View our secondary antibodies catalog for more detailed information.

FITC / TRITC fluorophore tagged secondary antibodies (1:4000 dilutions) was added for 1 hour at RT in dark.

Blots were developed with Goat anti-Rabbit IgG H&L (IRDye ® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye ® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20, 000 dilution for 1 hour at room temperature before imaging.

Proteintech offers a variety of secondary antibodies suitable for Western Blotting, ELISA, cellular imaging, and flow cytometry.

Secondary antibodies are often conjugated to substances that will enable the visualization of the primary antibody, such as HRP or fluorophores.

As part of the webinar series, Abcam would like to offer you a 50 % discount on the following product ranges: neuronal marker antibodies, Alexa Fluor ® conjugated secondary antibodies, agonists and antagonists, ion channel blockers and enzyme inhibitors and modulators.

The secondary antibodies were ab150115 Alexa Fluor ® 647 (red) goat anti-mouse IgG (H+L) used at 2µg / ml for 1h and ab150077 Alexa Fluor ® 488 (green) goat anti-rabbit IgG (H+L) used at 2µg / ml for 1h.

Fluorescent secondary antibodies (Table 3) were reacted with tissue sections for 2 h at room temperature before mounting with Fluoromount G (Southern Biotech).

Secondary antibodies were conjugated with horseradish peroxidase, and signals were developed using a chemiluminescent substrate (Super Signal West Pico, Thermo Scientific).