Sentences with phrase «secondary nanobody»
Antibodies were individually incubated with a secondary nanobody conjugated with Alexa Fluor 488, Alexa Fluor 568, or Alexa Fluor 647 before application to the sample.
http://blog.addgene.org/
Looking for primary monoclonal antibodies to use with the secondary nanobody toolbox?
Looking for monoclonal primary antibodies to use with the secondary nanobody toolbox?
Before picking a secondary nanobody, it's important to review a few characteristics of your primary antibody: species it was raised in and its IgG subclass.
A toolbox of anti — mouse and anti — rabbit IgG secondary nanobodies.
Instead, you can pre-incubate individual primary antibodies with secondary nanobodies conjugated with the desired fluorophores.
Enter the Görlich lab and their anti-mouse and - rabbit IgG secondary nanobodies toolbox.
Not exact matches
Nanobodies can be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (Pleiner et al figures 4A - C).
After this pre-incubation step, the primary antibody - nanobody mix can be added to your sample, thus eliminating the secondary antibody incubation.