Sentences with phrase «selectable marker»
A selectable marker is a gene or trait that helps in distinguishing between cells that have a desired characteristic or genetic modification, and cells that don't. It enables researchers to easily identify and select the cells they are interested in during experiments or genetic engineering. Full definition
Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics.
academicsreview.org
Other investigators who had been working to develop high efficiency gene targeting approaches improved on this finding with the addition of selectable markers that are only expressed when the targeting vector is inserted downstream of a functional promoter (gene traps).
The developed vector (1TAX) combined a triple combination of anti ‐ HIV genes (TRIM5, CCR5 shRNA, and a TAR decoy) and a truncated / mutated form of human CD25 (tCD25) as the cell surface selectable marker, in a self ‐ inactivating (SIN) third ‐ generation lentiviral vector.
Selectable marker genes in transgenic plants: applications, alternatives and biosafety.
In addition both constructs contain the constitutive Ubi promoter and nos T terminator, the bar gene conferring herbicide tolerance and may contain further sequences of bacterial origin including the neomycin phosphotransferase (nptI) selectable marker gene.
We previously described a method (Ni et al. 2008) whereby RNAi constructs are targeted into the genome by the phiC31 - mediated integration approach using Vermilion - AttB - Loxp - Intron - UAS - MCS (VALIUM), a vector that contains vermilion as a selectable marker, an attB sequence to allow for phiC31 - targeted integration at genomic attP landing sites, two pentamers of UAS, the hsp70 core promoter, a multiple cloning site, and two introns.
Digestion of the donor plasmids with SapI allows the tag, selectable marker, and connector to be released (Fig. 1, Step 2 - 3).
Our conclusion, supported by numerous studies, most of which are commissioned by some of the very parties that have taken a position against the use of antibiotic selectable marker gene systems, is that there is no scientific basis to argue against the use and presence of selectable marker genes as a class in transgenic plants.»
A landing - site cassette was created by flanking the selectable marker in pSK + - rpsL - kana [59] with 5» GAL4 and 3» HSP70 UTR arms from pBPGUw [60], a gift of G. Rubin.
The selectable marker can be removed by Cre - mediated excision for scarless tag insertion.
SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker -LRB-
SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr - unc - 119) for easy screening of the insertion event, and a variety of connector modules (linker sequences between the tag and homology arms).
If you are looking to co-express your gene of interest along with a fluorescent protein or selectable marker, it is easiest to start with a plasmid that already has the multicistronic element and reporter cloned in.
2fTGH is a human cell line containing the selectable marker guanine phosphoribosyltransferase regulated by alpha interferon (IFN - alpha).