Sentences with phrase «selection marker»

The plasmid was injected together with mCherry selection markers and CRISPR / Cas9 sgRNA plasmids.The sequence alignment and graphics were designed using SnapGene software.

Some transgenic crops contain antibiotic resistance genes as selection markers that were used during the process of transgene construction.

Furthermore, due to high efficiency of CRISPR / Cas9 system, cell colonies carrying multiple gene mutations can be isolated by limiting dilution and is free of selection maker, which avoids further removal of selection marker by recloning or intercrossing [35].

These vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains.