Collectively, these results indicate that our synthetic
sensing gene circuit shows ligand - dependent gene expression in plants.
Hence, we linked output of our complete synthetic
sensing gene circuit to the de-greening gene circuit by placing its genes (diRNA POR, protochlorophyllide oxidoreductase; AtChlase, chlorophyllase; AtRCCR, red chlorophyll catabolite reductase)[7] under control of the PlantPho promoter (Figure 1).
Response of transgenic tobacco plants with one copy of each gene circuit, de-greening gene circuit and synthetic
sensing gene circuit.
Figure S4 shows expression analysis of all components of
the sensing gene circuit (ss - TNT.
The sensing gene circuit is contained on a T - DNA providing resistance to Basta whereas the de-greening gene circuit is on a T - DNA providing resistance to kanamycin.
We first verified that
the sensing gene circuit, assembled from bacteria genes, was fully expressed in later plant generations.
Reverse transcriptase - polymerase chain reaction (RT - PCT) analysis of synthetic sensing and signaling components confirms expression of components of
the sensing gene circuit.
Not exact matches
Analogous to integrated
circuits that underlie myriad electronic products, engineered
gene circuits can be used to generate defined dynamics, rewire endogenous networks,
sense environmental stimuli, and produce valuable biomolecules.