Sentences with word «sepharose»

Aβ was immunoprecipitated from 50 μl CSF with 5 μg of the anti-Aβ antibody W0 - 2 (EMI Millipore, USA) coupled to protein - G sepharose beads (GE Healthcare, USA) and incubated overnight at 4 °C.

Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).

The next day, protein G sepharose was used to immunoprecipitate the RIPK2 protein complex immunoprecipitation (IP) for 1.5 hours.

For the in vitro GST pull - down assay, 20 μL of glutathione - Sepharose 4B beads (Amersham Pharmacia Biotech) with immobilized GST or GST / Sp1 fusion proteins (4 μg each) were incubated with HuH - 7 whole - cell extracts (500 μg) at 4 °C for 6 hours.

Signals observed in the non-transgenic animals and the PBS control result from either protein - G sepharose, the antibody or other non-specific binding components of the CSF.