Sentences with phrase «sequence a human genome using»

Not exact matches

Dec. 18, 2013 — The most complete sequence to date of the Neanderthal genome, using DNA extracted from a woman's toe bone that dates back 50,000 years, reveals a long history of interbreeding among at least four different types of early humans living in Europe and Asia at that time, according to University of California, Berkeley, scientists.

When the human genome draft was announced 10 years ago, many researchers and policymakers anticipated using the newly sequenced code to develop a wealth of diagnostic and treatment capabilities.

«We explored the opportunity of using sperm RNA elements as a predictor of human health, with applications at the fertility clinic that would go hand - in - hand with the new neonatal intensive care unit genome sequencing to better health outcomes,» said Dr. Krawetz, associate director of the C.S. Mott Center for Human Growth and Develophuman health, with applications at the fertility clinic that would go hand - in - hand with the new neonatal intensive care unit genome sequencing to better health outcomes,» said Dr. Krawetz, associate director of the C.S. Mott Center for Human Growth and DevelopHuman Growth and Development.

Using metabolic function analyses of identified genes, we compared our human genome with the average content of previously sequenced microbial genomes.

Using advanced sequencing technologies, University of Oklahoma anthropologists demonstrate that human DNA can be significantly enriched from dental calculus (calcified dental plaque) enabling the reconstruction of whole mitochondrial genomes for maternal ancestry analysis — an alternative to skeletal remains in ancient DNA investigations of human ancestry.

The goal of the human genome project was to use DNA sequencing to reveal all three billion DNA letters in our chromosomes and find all our genes.

They digested human genomic DNA using Cas9 nucleases in a test tube, which was then subjected by whole genome sequencing.

I always used to say we should treat the sequencing of the human genome like income tax: It's criminal to evade it, but there are legal means of avoiding it.

The millions of base pairs of sequence that Genovese and McCarroll's team have located will be added to the next release of the reference human genome assembly — the «Google maps» of the human genome that geneticists use every day — providing a more comprehensive view of the genome and how the pieces all fit together.

As when he worked on the human genome, Venter is relying on a radical technique called shotgun sequencing: He chops up vast amounts of DNA into tiny pieces and then uses sophisticated computer analyzers to piece them back together into intelligible genes and chromosomes.

In order to locate all gene switches, the Freiburg research team used modern sequencing methods to examine the entire genome — DNA, epigenetic markers and RNA — during the development, maturation and disease of human cardiac muscle cells.

The research team from the National Human Genome Research Institute (NHGRI) and the National Cancer Institute (NCI), both parts of NIH, extended their recent genome sequencing study of skin bacteria, using DNA sequencing techniques optimized for identifying Genome Research Institute (NHGRI) and the National Cancer Institute (NCI), both parts of NIH, extended their recent genome sequencing study of skin bacteria, using DNA sequencing techniques optimized for identifying genome sequencing study of skin bacteria, using DNA sequencing techniques optimized for identifying fungi.

Evolutionary geneticists Svante Pääbo, Johannes Krause, and colleagues at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, ground up a 30 - milligram sample and extracted and sequenced all of the 16,569 base pairs of its mtDNA genome, using new techniques Pääbo's group has successfully employed to sequence both Neandertal and prehistoric modern human DNA.

Over the next year, the company plans to use data from human genomes sequenced with its new machines to develop the statistics needed to evaluate how accurate the data are.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

Yet, «these studies using HeLa cells had to rely on information from the «reference» sequence produced by the Human Genome Project, even though there was evidence that the genomes of HeLa cell lines were probably quite different,» said Lars Steinmetz, Ph.D., who led the G3 study.

In 1991 he invented a quick way to find human genes, using expressed sequence tags, after the senior scientists at the Human Genome Project had said it wouldn't human genes, using expressed sequence tags, after the senior scientists at the Human Genome Project had said it wouldn't Human Genome Project had said it wouldn't work.

All the analysts and pundits were saying that genome sequencing was dead, there's no use for it anymore — the human genome has been sequenced.

The company's thermal cyclers were used by every major genome centre during the sequencing of the human genome and were most recently used by the BC Cancer Agency during the sequencing of the SARS virus.

The new NIST human genome RMs increase the ability of DNA sequencing laboratories to be more confident in their reporting of true positives, false positives, true negatives and false negatives, and therefore, significantly improve genetic tests used for disease risk prediction, diagnosis, and progression tracking.

Using a single pollinated pine seed, the team assembled the largest genome ever sequenced: 22.18 billion base pairs, more than seven times longer than the human genome.

Together with the French gene sequencing center Genoscope, in the Paris suburb of Evry, Celera will perform the initial sequencing using the «whole - genome shotgun» approach it unleashed on the human genome.

Using whole exome sequencing (a next generation test to analyze the exons or coding regions of thousands of genes simultaneously) conducted at the Baylor College of Medicine Human Genome Sequencing Center, the researchers identified CLP1 mutations in two unrelated families with thesequencing (a next generation test to analyze the exons or coding regions of thousands of genes simultaneously) conducted at the Baylor College of Medicine Human Genome Sequencing Center, the researchers identified CLP1 mutations in two unrelated families with theSequencing Center, the researchers identified CLP1 mutations in two unrelated families with the disorder.

Using the new technology, Complete Genomics» chief scientist Radoje Dramanac estimates that the company will soon be able to sequence a complete human genome in about a day.

In particular, the Neandertal genome sequence can now be used to catalog changes that have become «fixed» (are invariant within a population or species) in modern humans during the last few hundred thousand years and should be helpful for identifying genes affected by positive selection since humans diverged from Neandertals.

To go a step further, researchers are using more powerful genome - wide sequencing to take a detailed look at those viruses that resemble known threats to humans.

Today, the National Human Genome Research Institute (NHGRI) in Bethesda, Maryland, announced it will pour more than $ 116 million into three sequencing centers, which will use «large - scale» sequencing to focus on unraveling the sources of disease and elucidating the workings of disease - causing organisms.

Using a specific work flow, they assessed both the coding and noncoding regions of the human genome, including the evaluation of highly polymorphic SNPs, structural and copy number variations, as well as 69 control genomes sequenced by the same procedures.

Using the new approach in the hydatidiform genome, the researchers were able to identify and sequence 26,079 segments that were different from a standard human reference genome used in genome research.

For example, the large - scale sequencing program has already started to sequence the genomes of 100 microorganisms found in the human gut, and will build on this by using genomic sequencing to characterize the complex microbial communities found at many sites in and on the human body.

In order to locate all gene switches, the Freiburg research team used modern sequencing methods to examine the entire genome - DNA, epigenetic markers and RNA - during the development, maturation and disease of human cardiac muscle cells.

NORMAN — Using advanced sequencing technologies, University of Oklahoma anthropologists demonstrate that human DNA can be significantly enriched from dental calculus (calcified dental plaque) enabling the reconstruction of whole mitochondrial genomes for maternal ancestry analysis — an alternative to skeletal remains in ancient DNA investigations of human ancestry.

We will use a lab - adapted human strain of rotavirus with a known genome sequence to test and optimize this sequence - independent amplification method.

Chief among the restrictions that accompanied the X Ten was the requirement that X Tens only be used for whole - genome sequencing, and only for human genomes.

This flash animation shows the processes involved in the Sanger sequencing method — the DNA sequencing method used during the Human Genome Project.

I will review recent advances in the use of nanopore sequencing for clinical microbiology and human genetics, including our collaborations on viral and bacterial diagnostic sequencing, real - time surveillance, direct RNA and human whole - genome sequencing, and discuss the opportunities and barriers around moving to sequencing as a routine test in the clinic.

The Cancer Genome Project uses human genome sequence and mutation detection techniques to find changes in DNA involved in the development of human caGenome Project uses human genome sequence and mutation detection techniques to find changes in DNA involved in the development of human cagenome sequence and mutation detection techniques to find changes in DNA involved in the development of human cancers.

The Cancer Genome Project at the Wellcome Trust Sanger Institute is part of an international project using DNA sequencing to identify mutations in genes critical to the development of human cancers.

With the effort to sequence the entire human genome speeding toward completion, some researchers are now focusing their energy on developing the next generation of tools that can be used to extract...

Biological Annotation of the Genomic Sequence A key use of the sequence information from the canonical model organisms, such as Drosophila, will be to help interpret the sequence of the humanSequence A key use of the sequence information from the canonical model organisms, such as Drosophila, will be to help interpret the sequence of the humansequence information from the canonical model organisms, such as Drosophila, will be to help interpret the sequence of the humansequence of the human genome.

The use of higher resolution techniques, such as array CGH and, soon, whole genome sequencing, will enhance the ability of researchers to examine stem cell lines to determine which are best — least likely to result in diseases and other problems — to use in creating therapies for use in humans.

We use genome sequences to advance understanding of the biology of humans and pathogens to improve human health

Here at the Wellcome Sanger Institute, we use genome sequences to better understand human and pathogen biology to improve human health.

A few interesting articles in early life human microbiome, plus: A comparison between Staphylococcus epidermidis commensal and pathogenic lineages from the skin of healthy individuals living in North American and India; A new tool to reconstruct microbial genome - scale metabolic models (GSMMs) from their genome sequence; The seasonal changes in Amazon rainforest soil microbiome are associated with changes in the canopy; A specific class of chemicals secreted by birds modulates their feather microbiome; chronic stress alters gut microbiota and triggers a specific immune response in a mouse model of colitis; and evidence that the short chain fatty acids profile in the gut reflects the impact of dietary fibre on the microbiome using the PolyFermS continuous intestinal fermentation model.

Using its proprietary sequencing instruments, chemistry, and software, the company has sequenced more than 20,000 whole human genomes.

We have a variety of data, both from actual assemblies and simulation studies, which show that about 96 % of the reference human genome is addressable using this library and sequencing strategy, including a significant fraction of the high - copy repeat sequences in the genome.

Tracing the Route of Modern Humans out of Africa by Using 225 Human Genome Sequences from Ethiopians and Egyptians.

Very good for giving the overview of the BioTech industry as it applies to the Human Genome sequencing race and the potential for use of this information in the future for Personalized Medicine.

ChIP - Seq sequencing reads were mapped to the human genome (GRCh37 / hg19) using Bowtie2 with default parameters (v2.1.0, [27]-RRB-.

By the mid-1990s, when the Human Genome Project was in full swing, scientists were sequencing DNA using capillary sequencers.