Sentences with phrase «sequence at the target site»
When Piwis recognize their target, they recruit a set of even smaller secondary RNAs corresponding to the sequence at the target site, a way of marking it for attention.
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Cell growth was monitored every 48 hours post-stimulation for approximately two weeks and the efficiency of CXCR4 disruption was assessed at day five post-transduction by both the Surveyor nuclease assay and by deep - sequencing of the CXCR4 target site.
The advancement of the Cas9 - based platform for screening and validation will help further the development of new therapeutic products, and Caribou's CRISPR - Cas9 technology can utilize guide RNAs specific for unique sequences and target a gene at numerous sites and therefore provide enhanced specificity.
The potential off - target sites were selected based on the following rules: (1) the protospacer - adjacent motif (PAM) sequence is NGG; (2) the homology with the 12 base «seed sequence» at the 3 ′ end of the gRNA [6].
These potential off - target sites were analyzed by DNA sequencing, but no unwanted mutation occurred at these genomic sites in all cloned goats (Table S2).
(B) Schematic representation of the pCMV - Neo-APC expression vector that codes the full - length APC sequence together with wild - type or mutated miR - 142 targeted site located at the 3 ′ UTR.