A system for automated detection of homologs among the annotated genes of several completely
sequenced eukaryotic genomes.
Not exact matches
Surprises include the discovery of about 20
eukaryotic genes; other bacteria that have been
sequenced have had few, if any, such genes.
Our current concept of the origin of the
eukaryotic cell is in flux, however, and an evolutionary
sequence that appears simple when conceptualized on a phylogenetic tree diagram may be far more complex and interesting in reality.
The EBP would focus on the natural world, providing a better understanding of biodiversity by first
sequencing in great detail the DNA of a member of each
eukaryotic family (about 9000 in all) and eventually generating coarser genomes for the other eukaryotes.
The Gordon and Betty Moore Foundation accepted
sequencing nominations from Dr. Worden and provided
sequencing funds in support of understanding
eukaryotic algae.
«The absence of
sequences closely related to the slowly changing proteins of the
eukaryotic cytoskeleton remains unsettling,» Dr. Russell Doolittle of the University of California, San Diego, wrote in the March 26 issue of Nature.
Our method, based ideally on 20x and 50x of NaS and Illumina reads respectively, provides an efficient and cost - effective way of
sequencing microbial or small
eukaryotic genomes in a very short time even in small facilities.
As is often the case for yeast, the ability to
sequence and analyze whole genomes at very deep coverage has yielded broad insights on
eukaryotic genome evolution.
The technology included a variety of expression systems, including the wheat germ cell free translation system for
eukaryotic gene expression, to take synthetic gene
sequences and translate them into proteins.
The random BAC fingerprinting technique has rapidly become the method of choice for mapping and
sequencing the comparatively large genomes of other
eukaryotic organisms, including humans.
Internal initiation of translation of
eukaryotic mRNA directed by a
sequence derived from poliovirus RNA.
IRES was originally discovered in poliovirus RNA, where it promotes translation of the viral genome in
eukaryotic cells.1, 2 Since then, a variety of IRES
sequences have been discovered - many from viruses, but also some from cellular mRNAs.
The ability to quickly obtain accurate genome
sequences of
eukaryotic pathogens at low costs provides a tremendous opportunity to identify novel targets for therapeutics, develop pesticides with increased target specificity and breed for resistance in food crops.
Oxford Nanopore
sequencing, hybrid error correction, and de novo assembly of a
eukaryotic genome
For
eukaryotic proteins (excluding plant
sequences) an overall prediction accuracy of 66 % for four locations was achieved, with 33 % of the
sequences being predicted with an accuracy of 82 % or better.
With the current
sequencing technology and bioinformatics tool set, we are able to deliver an
eukaryotic fungal genome at low cost within a week.
Here, we present the first report of the ~ 54 MB
eukaryotic genome
sequence of Rhizoctonia solani, an important pathogenic fungal species of maize, using nanopore technology.
We have
sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large
eukaryotic genomes.
Using MinION nanopore
sequencing to generate a de novo
eukaryotic draft genome: preliminary physiological and genomic description of the extremophilic red alga Galdieria sulphuraria strain SAG 107.79
The test addresses the following areas: - difference between living and nonliving things - semipermeable membrane - the
sequence of increasing or decreasing levels of organization of the human body - the cell theory - Hooke and Leeuwenhoek - four common structures found in all cells - prokaryotic vs.
eukaryotic cells - functions and structure of microscopes - cell organelles - difference between plant and animal cells - procedure for making a wet - mounted slide