Sentences with phrase «sequencing approach used»

The high - intensity sequencing approach used in this study is a research platform and is not intended to be commercially available to patients.

The high - intensity sequencing approach used in this study has a unique combination of breadth and depth.

Not exact matches

The CRISPR approach is very good at disabling genes, but using the technique to fix them is much harder, because it involves replacing a faulty sequence with another.

This new sequencing approach can be used for any DNA that is too fragmented to be read well through more traditional methods.

Using the genetic data, along with high - quality 3D photographs of the participants» faces, the researchers used an artificial intelligence approach to find small differences in DNA sequences, called SNPs, associated with facial features such as cheekbone height.

«By using this approach, we first tried identify the structural modules of well - studied proteins by using as input solely their sequence information and using statistical inference methods.

Rubin's used a metagenomic approach, incorporating fragments of Neandertal DNA into loops called plasmids, amplifying them in bacteria and then using known sequences of human DNA to isolate strands for sequencing.

«Depending on the research questions, it may also be important to define the appropriate strategy to use, either whole - genome sequencing or just targeting some genomic regions with a gene baiting approach,» says Besnard.

«However, our new approach uses cutting - edge DNA sequencing technology to precisely measure the effects of thousands of sequences on gene activity at the same time.»

Using their innovative approach, Emsley's team found that the sequence of protein motions follows a specific hierarchy as temperature increases: first the protein's solvent molecules, then the protein's side - chains and water molecules, and finally the protein's backbone.

Described in a study published in the journal Nature Communications, this novel approach uses high - speed atomic force microscopy (AFM) combined with a CRISPR - based chemical barcoding technique to map DNA nearly as accurately as DNA sequencing while processing large sections of the genome at a much faster rate.

Gardy's team used whole - genome sequencing to track the outbreak, an approach they pioneered four years ago when analyzing TB outbreaks.

Using this approach, we have sequenced ~ 14,000 protein - coding positions inferred to have changed on the human lineage since the last common ancestor shared with chimpanzees.

To trace the source of the anthrax, the FBI used «microbial forensics,» a new approach that combines established microbiology techniques with DNA sequencing.

To overcome the extreme genomic complexity, the team used new long - read sequencing technology that boosted the quality of the genome sequence obtained by more than one hundred fold over standard short - read approaches.

The team used a new approach to gene sequencing that enabled them to completely reconstruct the virus» previously unknown genome in less than 24 hours — significantly faster than conventional sequencing techniques, which can take days to weeks.

«For our study, we examined 19 autonomous adenomas using whole exome sequencing,» Calebiro describes their approach.

In a second Nature paper, also published online today, another group of researchers used a different genetic sequencing approach, and a different set of mammals, to ask similar questions about the evolution of the Y chromosome.

This is the first study in human trials to identify genes driving acute peanut allergic reactions using a double - blind placebo - controlled approach with comprehensive sequencing of genes expressed before, during, and after they ingested peanut.

This innovative approach — using high - intensity sequencing to detect cancer from circulating tumor DNA in the bloodstream — heralds the development of future tests for early cancer detection.

While circulating tumor DNA tests targeting a smaller set of cancer genes are already available for use in routine practice to guide care, by covering a much larger number of cancer genes, this high - intensity sequencing approach may enable development of future tests for early detection of cancer.

In the new ramp control approach, a computerized algorithm is used to calculate the two - dose sequence of anesthetic administration likely to produce sedation in each individual patient.

Together with the French gene sequencing center Genoscope, in the Paris suburb of Evry, Celera will perform the initial sequencing using the «whole - genome shotgun» approach it unleashed on the human genome.

Because the protein sequence is directly related to the DNA code, the team says that comparing protein sequences — like comparing DNA sequences — is an approach that can be used to determine degrees of relatedness between species and to decipher how ancient animals evolved.

Using the new approach in the hydatidiform genome, the researchers were able to identify and sequence 26,079 segments that were different from a standard human reference genome used in genome research.

Using this «Ribotag» approach tissue - specific changes under DR have been measured in muscle, fat, gut, heart, neurons, germline and malpighian tubules, showing a highly tissue specific response upon DR.. We are developing mechanistic models of gene expression regulation and conducting comprehensive bioinformatics analyses on UTRs, promoters and the coding sequences of various elements of differentially regulated genes.

This approach avoids the complex and unreliable workflows associated with culture of bacteria or amplification of DNA and draws on the remarkable throughput and ease of use of modern sequencing approaches.

Our approach uses metagenomic DNA to construct large ‐ scale heterologous expression libraries that are tracked over time in vivo by deep sequencing and computational methods.

We use next - generation sequencing to analyze circulating and shed biomarkers in order to develop better approaches for primary, secondary, and tertiary ovarian cancer prevention.

A large variety of methods is used including genetic, molecular and cytogenetic techniques, fluorescence in situ hybridisation and DNA «combing», as well as high throughput sequencing approaches such as DNA - seq for mutation landscape analyses, RNA - seq for transcriptome analyses and ChIP - seq mapping of chromatin - associated proteins and their genome - wide modulation in response to DNA damages.

And, while the genetically - modified mice were made somewhat better by deactivating the HD gene in their hypothalamus, this approach isn't useable in human HD patients because their HD genes don't contain the sequences needed to turn them off with virus used by Petersen and colleagues.

Conclusions We described NaS tool, a hybrid approach allowing the sequencing of microbial genomes using the MinION ® device.

Strain - specific search of pathways obtained from multiple sources using various approaches (e.g. manual curation, automatic assignment by sequence similarity, etc).

Therefore, I am engaged in using, developing and combining new technologies, including: (1) super-resolution microscopy, (2) single molecule imaging, (3) genetic engineering, (4) next generation sequencing approaches and (5) mathematical and biophysical modeling.

She also pioneered the use of DNA sequencing for human rights investigations, developing the approach of sequencing mitochondrial DNA preserved in human remains, then applying this method to the identification of kidnapped children in Argentina and subsequently to cases of human rights violations on six continents.

Therefore, Teitell's group opted to further modify their constructs with a 3 ′ - UTR mitochondrial targeting sequence (MTS) from human mitochondrial ribosomal protein S12 — the same essential approach used to optimize the import of allotopically - expressed proteins by Dr. Marisol Corral - Debrinski, (3,4) and subsequently advanced for multiple additional ETS subunit proteins by Dr. Matthew O'Connor's group at the SENS Foundation Research Center (RC).

We use a combined experimental and computational approach to predict whether sequence motifs and / or pre-existing histone modifications direct the recruitment of histone methyltransferases to their targets.

Professor Nick Thomson, from the Wellcome Trust Sanger Institute and the London School of Hygiene & Tropical Medicine (LSHTM), is part of the team that are using new DNA sequencing approaches to better understand how this disease is spread.

Proprietary genome assembly pipeline using shotgun genome sequencing and / or fosmid pool approaches

«A huge fraction of the human «proteome» remains uncharacterized, and this paper shows how chemical approaches can be used to uncover proteins of a given functionality that have eluded classification based on sequence or predicted structure,» said co-senior author Benjamin F. Cravatt, chair of TSRI's Department of Chemical Physiology.

An in vivo - vitro shuttle system for the study of P. cynomolgi liver stages12 is being exploited to better understand hypnozoite biology using molecular tools and genome - scale approaches, which rely on the availability of a complete and well annotated P. cynomolgi reference genome sequence.

We focus on squamous tissues, the skin epidermis and the lining of the oesophagus, using transgenic models, novel sequencing approaches, live imaging and single cell analysis to uncover key steps in cancer development, with aim of developing rational interventions to decrease cancer risk.

It will make use of cutting - edge approaches in vivo and in vitro, including mouse genetic models and next - generation sequencing of RNA and immunoprecipitated chromatin (RNA - seq and ChIP - seq).

Our work extends over these different aspects, through the use of fine developmental and molecular dissection, combined with genetic tools (CRISPR) and genome - wide sequencing approaches.

Among vertebrates, the fish model displays easy high density breeding (short generation time), favourable biological peculiarities (accessibility and transparency of the eggs, genome sequenced for both species used on the platform), original technological approaches (confocal and two - photon microscopy), as well as a wide accessibility to fully transparent larvae usable up to 5 days after fertilization.

Klein will investigate, using the genotyping by sequencing approach, the chromosomal location of potential genetic barriers affecting successful hybridization between the two.

Supervises wet lab, building amplicon, genomic and metagenomic libraries using various approaches to sequence on variety of platforms (illumina as well as PacBio) to study the understanding of the relationship between the microbiome and metabolic diseases.

Rapidly evolving approaches to the comprehensive profiling of patients» risk factors, such as whole - genome sequencing, will play an increasingly important role in overcoming neurological disease, in much the same way as these approaches are already used to match up certain cancer patients with specific drugs they stand to benefit from the most.

Economic Modeling: Our economic modeling core, led by Dr. Ann Wu, uses a variety of modeling approaches to project the medical and financial consequences of genomic medicine, including the use of pharmacogenomic testing to inform medication choices and the use of sequencing as an adjunct to standard newborn screening.

This section invites manuscripts describing (a) Linkage, association, substitution or positional mapping and epigenetic studies in any species; (b) Validation studies of candidate genes using genetically - engineered mutant model organisms; (c) Studies focused on epistatis and gene - environment interactions; (d) Analysis of the functional implications of genomic sequence variation and aim to attach physiological or pharmacogenomic relevance to alterations in genes or proteins; (e) Studies of DNA copy number variants, non-coding RNA, genome deletions, insertions, duplications and other single nucleotide polymorphisms and their relevance to physiology or pharmacology in humans or model organisms, in vitro or in vivo; and (f) Theoretical approaches to analysis of sequence variation.