The researchers tested the drug on mice with H. pylori infections from
several different cell lines, and found that the drug was effective against the H. pylori while maintaining populations of healthy gut bacteria.
Even then, with a completely clean lab highly focused on meticulous technique, a new contamination struck in 2013 — something Myers identified when
several different cell lines showed up with exactly the same mutation.
Not exact matches
Several different experiments localized the active gene and its protein to stem
cells buried within the crypts, or deep grooves in the intestinal
lining.
The authors studied a standard panel of 60 established human tumor
cell lines representing nine
different human cancers, as well as
several specimens of human primary ovarian cancer.
The overall score encompass
several factors: reproducibility of the antibody staining in
different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to
different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.