A guide to light -
sheet fluorescence microscopy for multiscale imaging.
During his PhD he worked on three main projects: digital scanned laser light
sheet fluorescence microscopy (with Dr. Ernst Stelzer), the in toto reconstruction of zebrafish embryogenesis (with Dr. Jochen Wittbrodt), and the computational analysis of the evolution of the yeast genome architecture (with Dr. Michael Knop).
Modern light
sheet fluorescence microscopy was first pioneered by Voie and colleagues and originally named orthogonal - plane fluorescence optical sectioning (OPFOS)(2).
Not exact matches
Acquiring images using modern techniques such as light
sheet fluorescence, confocal, or electron
microscopy creates a significant data stream.
These include custom - built light
sheet microscopy for single molecule and whole embryo measurements, laser ablation to perturb and dissect the architecture of microtubule structures, quantitative polarization
microscopy, and new
fluorescence correlation spectroscopy approaches.
In 2015 light
sheet - based
fluorescence microscopy was honoured as «Method of the Year 2014» by Nature Methods.
Since last year, LUXENDO is a part of the BRUKER corporation, adding light
sheet microscopy to BRUKERs
fluorescence microscopy branch.
Overcoming the limitations of confocal
fluorescence microscopy, we invented and developed light
sheet - based
fluorescence microscopy (LSFM) establishing it as a major tool in the modern life sciences.
Fully developed and drug - affected several hundred µm thick spheroids are not very dynamic but require confocal and light
sheet - based
fluorescence microscopy for a full analysis of the spatial distribution of several fluorescing targets.