A contribution by Matyas Molnar, displaying a light
sheet microscopy image of a bug head, was voted the favorite by the conference participants.
Not exact matches
While light -
sheet microscopy is an old idea — scientists at ZEISS
Microscopy and collaborators first came up with it in 1903 — only in this century has the convergence of fluorescent labels that work to process
image volumes combined to make light -
sheet mainstream.
Acquiring
images using modern techniques such as light
sheet fluorescence, confocal, or electron
microscopy creates a significant data stream.
Simultaneous multiview light -
sheet microscopy (see
image, «PACKING IT IN,» in slideshow above) uses thicker
sheets to track ensembles of cells.
Since its development, lattice light -
sheet microscopy has been used to
image numerous important events, such as single transcription factor molecules binding to DNA, hotspots of transcription, microtubule instability, protein distributions in embryos, and much more.
Although traditional in vivo imaging tools, such as widefield and confocal
microscopy, and newer ones, such as light -
sheet microscopy, can
image in three dimensions, they sacrifice substantial spatiotemporal resolution to do so and, even then, can often be used for only very limited durations before altering the physiological state of the specimen.
The combination of two processes makes this high - resolution 3D imaging possible: lattice light -
sheet microscopy (LLSM), which
images one slice of the cell at a time, and adaptive optics (AO), which corrects for any blurriness.
Super-resolutive light
microscopy (PALM / STORM), light
sheet microscopy and advanced expertise for
image analysis are available.
In theory, light
sheet microscopy should be quicker than snapping
images from spot to spot within a plane.