A
"short hairpin" refers to a small, U-shaped piece of metal or other material that is used to hold hair in place. It is typically shorter in length compared to regular hairpins.
Full definition
Then, in Goga's UCSF laboratory, thousands of
short hairpin RNAs (shRNAs), bits of genetic material that precisely shut down the activity of specific proteins, were sequentially introduced to the engineered cells — in this case, shRNAs targeting proteins called kinases, a common target of cancer drugs, were used.
The discovery
of short hairpin RNAs (shRNAs) suppressing gene expression in mammalian cells enables large - scale loss - of - function screens by using genome - wide shRNA libraries.
We have devised a massively parallel strategy for
screening short hairpin RNA (shRNA) collections for stable loss - of - function phenotypes.
The researchers then developed a new type of single - strand RNA interference (RNAi) agent, the proline -
modified short hairpin RNA (PshRNA), which suppresses gene expression by utilizing the cellular system.
Cold Spring Harbor Laboratory engaged Hairpin Technologies Inc. to expand the commercial distribution and research use
of short hairpin RNA...
The main analysis, of 10,000
short hairpin RNAs, targeted about 3000 different genes, says Elledge.
Here, the researchers delivered a therapeutic gene along with
a short hairpin RNA (shRNA) into liver cells in mice.
My Honours project focused on genetically validating the Signal Transducer and Activator of Transcription 3 (STAT3) protein as a therapeutic target by using a novel transgenetic mouse model in which
a short hairpin RNA directed against STAT3 can reversibly be induced by the administration of doxycycline.
Finally, knocking down CD44 with
a short hairpin RNA produced the same results as treating cells with miR - 34a did — reduced tumor development, tumor burden and metastases.
Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (
short hairpin) RNA libraries for our experiments.