This was a two - part project: first, we used
shotgun sequencing data to identify SNPs in a few recombinant inbred lines (RILs).
Not exact matches
«Scientists challenge next - generation
sequencing dogma: Surprising bacteria study reveals that
shotgun sequencing misses major groups of organisms despite producing more
data than amplicon
sequencing.»
The goals of the project are as follows: (1) to
shotgun sequence the C. murisgenome to 8X coverage; (2) to
sequence 20,000 clones from a C. muris cDNA library to provide gene prediction training and verification
data; and (3) to manually annotate the C. muris genome
sequence in comparison with the genome
sequence data for C. parvum and C. hominis.
Since its introduction a decade ago, whole - genome
shotgun sequencing (WGS) has been the main approach for producing cost - effective and high - quality genome
sequence data.
This «scaffold
sequence» is being used to assist in assembly and finishing of the whole - genome
shotgun data.