Sentences with phrase «signal peptide»
A "signal peptide" is a short sequence of amino acids found at the beginning of a protein. It acts like a signal or label that guides the protein to the right place within a cell. Full definition
A suite of signaling peptides and their receptors regulate tissue stability as the pollen tube navigates toward the ovule.
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We re-engineered the DHP8 Trg - PhoR fusion for plant expression by adding an N - terminal signal peptide from a protein with known cell membrane localization (FLS2 [16]-RRB- to produce Fls - Trg - PhoR.
Addition of a plant signal peptide targets computationally re-designed receptors to the plant apoplast.
Although the reasons why Plasmodium proteins are difficult to express in heterologous expression systems are not clear, several protein characteristics, such as high molecular mass (> 60 kDa), presence of export motifs, and atypical signal peptide sequences negatively impact recombinant expression [12].
Protein coding sequences were flanked with unique NotI and AscI restriction sites and subcloned into a derivative of the pTT3 expression plasmid between a 5» mouse variable κ light chain signal peptide [32], and a 3» tag consisting of the rat Cd4 domains 3 and 4 followed by an enzymatic biotinylation sequence and a hexahistidine tag [33].
Unlike its ErmBL counterpart, the ErmCL signal peptide interacts directly with the antibiotic.
«Since there are other signal peptides apart from the ErmBL leader, we were interested in finding out whether they make use of this same mechanism or have different modes of action,» Wilson explains.
However, as Wilson explains: «The genes necessary for resistance are often activated only when required (i.e., when the antibiotic is present in the environment), and so - called leader or signal peptides play an important role in this process.»
They are responsible for adding sugar molecules to numerous different types of proteins, whether it's a cell wall, or it's a small signaling peptide, which is what's responsible for the phenotypes in tomato.»
Prior to the new study, scientists thought that the machinery in a cell created signalling peptides by cutting fragments out of proteins in sequence, and displaying these in order on the surface of the cell.
RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture media.
The secretory signal peptide is a ubiquitous protein - sorting signal that targets its passenger protein for translocation across the endoplasmic reticulum membrane in eukaryotes and the cytoplasmic membrane in prokaryotes.
Protein sequences from 3D7 reference P. falciparum strain were truncated to remove endogenous signal peptides and sequences corresponding to GPI anchors.
«SIGNAL PEPTIDES ™ Firming Serum harnesses the power of all - natural sunflower - derived lipids, rice peptides, and linseed extract to redefine facial contours.»
Once larvae feed, the fat body produces a number of signaling peptides, including growth factors and cytokines.
Briefly, full - length secreted molecules and the entire ectodomains of membrane - embedded proteins were identified using transmembrane [28], GPI - anchor [29], and signal peptide [30] prediction software.
Instead, the signal peptide adopts a specific conformation in the presence of the antibiotic, which blocks and inhibits the active center of the ribosome, thus accounting for the premature stop.
The researchers had previously shown that the signal peptide encoded by the ermBL mRNA does not interact directly with erythromycin.
A number of proteins do not have signal peptides (called leaderless proteins), and their secretory pathway have not been elucidated thus far.
Signal peptides are short peptides in proteins through which proteins are bound for specific cellular distribution or secretion.
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and / or transmembrane regions (orange) are predicted by MDM, these are displayed.
Sven Klose (Knust, MPG)-- «Investigation of the signal peptide and establishment of a novel structure - function analysis of crumbs in Drosophila melanogaster» (2011)
We replaced the bacterial periplasmic signal peptide from RBP with a plant signal peptide.
The signal peptide from the Arabidopsis FLS2 gene (At5g46330)(16) was fused to the start codon of Trg - PhoR, cloned into a pBS - PNOS - TNOS - TB intermediate vector, and sub-cloned into pCB302 - 3.
We thank Pat Bedinger for a signal peptide plasmid, ASN Reddy for a FLS2 leader plasmid, Marinus Pilon for use of his FluorCam and Keira Havens for manuscript help.
Most well - characterized effector molecules in other insects are secreted into the hemolymph: in our study, 91.2 % of previously identified AMPs have bioinformatic evidence indicating presence of a signal peptide.
Overall, 86.3 % (139/161) of induced genes that have no homology - based evidence for an immune function are either members of multi-gene families, encode proteins shorter than 300 amino acids, encode proteins with bioinformatic evidence for a signal peptide, or have a combination of these properties.
In a similar fashion, other tools assess whether mutations fall within annotated Pfam domains or signaling peptides of the encoded protein.
This cream is loaded with amino acids, signal peptides, and safe, synthetic growth factors, and it.