Sentences with phrase «signal peptide sequences»

Although the reasons why Plasmodium proteins are difficult to express in heterologous expression systems are not clear, several protein characteristics, such as high molecular mass (> 60 kDa), presence of export motifs, and atypical signal peptide sequences negatively impact recombinant expression [12].

Not exact matches

Prior to the new study, scientists thought that the machinery in a cell created signalling peptides by cutting fragments out of proteins in sequence, and displaying these in order on the surface of the cell.

Peptide quantification is facilitated by associating signal intensities in features with peptide sequences derived from MS2 sPeptide quantification is facilitated by associating signal intensities in features with peptide sequences derived from MS2 speptide sequences derived from MS2 spectra.

Protein sequences from 3D7 reference P. falciparum strain were truncated to remove endogenous signal peptides and sequences corresponding to GPI anchors.

Protein coding sequences were flanked with unique NotI and AscI restriction sites and subcloned into a derivative of the pTT3 expression plasmid between a 5» mouse variable κ light chain signal peptide [32], and a 3» tag consisting of the rat Cd4 domains 3 and 4 followed by an enzymatic biotinylation sequence and a hexahistidine tag [33].