With refinement of
signal transduction components, stronger and more specific signaling pathways may be possible.
We show that evolutionarily conserved histidine kinase
signal transduction components [9], [10], [11] can be used to build a synthetic signal transduction system, and we then use this system to link input from computationally re-designed receptors to a plant response.
While the threshold - like behavior of the de-greening circuit likely contributes to these differences, we can not rule out the need to balance our synthetic HK
signal transduction components for optimal transmission, in a manner similar to that in bacteria [31].
Not exact matches
Clara Franzini — Armstrong writes about Annemarie Weber, «a major contributor to the renaissance of muscle biology research in the 1950s to 1970s, when the
components of the contractile machinery were identified; novel views of muscle contraction and regulation were elucidated; and principles of energy
transduction, motility and intracellular
signaling common to all cells were revealed.»
Cbl - b is an E3 ubiquitin ligase that targets multiple
components of the TCR
signal transduction pathway for degradation through the proteasome to facilitate termination of TCR
signaling (19, 20).
Phosphotransfer - mediated
signaling pathways, involved in
signal transduction, have been identified in Pseudomonas as the two -
component GacS / GacA global regulatory system (39).
Figure 3A shows results of four control experiments, consisting of transgenic plants that lack one
component of the complete
signal transduction system (lack the receptor, lack the transmembrane HK, or lack the modified response regulator) or in which the critical phospho - accepting Asp53 residue was mutated.
In addition, because we developed PhoB - VP64 for plants by activating endogenous HKs with exogenous cytokinins [12], it is likely that our synthetic
signal transduction system (receptor → HK → PhoB: VP64 →
signal receptive promoter) has false input or cross-talk from plant HK
components.
Plant HK
components are involved in hormonal and possibly light - mediated
signal transduction processes, hence it is difficult to eliminate their input in our system.
One mechanism could be to differentially regulate the molecular
components involved in cue detection, such as ORs or VRs [20] or in known elements of their
signal transduction pathways, under the control of sex - specific hormones.