Sentences with phrase «single allele»

Locus - Specific Enhancer Hubs And Architectural Loop Collisions Uncovered From Single Allele DNA Topologies

«As it has only one set of chromosomes, it is very easy to find interesting mutants as all you have to do is to alter a single allele to produce a phenotype,» explains Oscar Fernández - Capetillo, head of the Genomic Instability Group and the leader of the research project.

Seventeen of the subjects in Bertolino's pool carried the SNPs on a single allele and showed increased activity in their striata and prefrontal cortices during the mental exercises, yet performed worse on the memory tests and had less attention control than the other study participants.

Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.

First, unlike CRISPR, the AAV - based approach is only able to target a single allele of a single gene at once.

Because patient XPCS2 was a hemizygote with mutant XPD protein (XPDG602D) expressed from a single allele, the corresponding mutation was expected to be viable in the homozygous state.

For comparison, in 2007 it took about 3 months to knockout a single allele in a human line, using methods based on homologous recombination (Rago et al., 2007).