Sentences with phrase «single cell genome»

«Multiclonal invasion identified in early breast tumors by topographic single cell genome sequencing»

Not exact matches

In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.

Eventually, researchers like Maley would like to examine whole genomes in single cells.

Another is how cells in a single organism take on different functions despite having identical genomes.

«We feel it's critical that the scientific community consider the potential hazards of all off - target mutations caused by CRISPR, including single nucleotide mutations and mutations in non-coding regions of the genome,» says co-author Stephen Tsang, MD, PhD, the Laszlo T. Bito Associate Professor of Ophthalmology and associate professor of pathology and cell biology at Columbia University Medical Center, and in Columbia's Institute of Genomic Medicine and the Institute of Human Nutrition.

«Just as normal cells with the same genome differentiate into many different cell types, a single tumor characterized by specific genetic mutations can contain many different types of cells — stem - like and more differentiated cells — with the difference being rooted in their epigenetic information.

Although researchers do not yet know the biological significance of these discoveries, they say that fully cataloguing the genome may help them understand how genetic variations affect the risk of contracting diseases such as cancer as well as how humans grow from a single - celled embryo into an adult.

Recent reports have found that conventional cell isolation and genome amplification strategies, even using MDA, can yield as many as a million false positive single - nucleotide variants in a human genome, swamping the true positives (1).

«Whole - genome, single - cell [sequencing] costs about the same as bulk - cell sequencing,» says BGI's Peng.

The diversity of genome amplification options underscores the importance of having a clear experimental question in mind before starting a single - cell sequencing project.

The TruePrime Single - Cell Whole Genome Amplification (WGA) kit uses a revolutionary multiple displacement amplification method based on the combination of the recently discovered DNA primase Thermus thermophilus (Tth) PrimPol and the extremely processive, high - fidelity Phi29 DNA polymerase (Phi29 DNAP) to amplify uniformly total genomic DNA of either a single cell or a few Single - Cell Whole Genome Amplification (WGA) kit uses a revolutionary multiple displacement amplification method based on the combination of the recently discovered DNA primase Thermus thermophilus (Tth) PrimPol and the extremely processive, high - fidelity Phi29 DNA polymerase (Phi29 DNAP) to amplify uniformly total genomic DNA of either a single cell or a few ceCell Whole Genome Amplification (WGA) kit uses a revolutionary multiple displacement amplification method based on the combination of the recently discovered DNA primase Thermus thermophilus (Tth) PrimPol and the extremely processive, high - fidelity Phi29 DNA polymerase (Phi29 DNAP) to amplify uniformly total genomic DNA of either a single cell or a few single cell or a few cecell or a few cells.

Mitochondria carry only a few genes, but they are so plentiful that it's often easier to find their DNA than the single full human genome in a cell's nucleus.

«Whole - genome, single - cell [sequencing] costs about the same as bulk - cell sequencing.»

The result was an extremely high - throughput sequencing protocol that, in a single run, can cover portions of the genomes of over 50,000 microbial cells (2).

«Animal viruses typically have all genome segments packaged together into a single viral particle, so only one of those particles is needed to infect a host cell,» Ladner explained.

Because T. vaginalis is a tiny single - celled protozoan that must hijack human cells to survive, it was expected to have a rather paltry genome.

The genomes of these 377 bacterial isolates, plus an additional 107 single bacterial cells from roots of A. thaliana, were then sequenced, assembled, and annotated at the JGI.

«Single - cell transcriptogenomics will be instrumental in gaining a more complete understanding of how variations in the genome can lead to functional deficiencies in aging and disease.»

Just as no two people possess the same genetic makeup, a recent study has shown that no two single tumor cells in breast cancer patients have an identical genome.

Navin's team developed Nuc - Sec as a single - cell genome sequencing method and applied it to study how cell mutations occur in both types of breast cancer.

The specificity of the approach enabled the researchers to visualize the delivery of the eight influenza genome segments to the cell nucleus where the virus replicates, and to analyze co-infections by two influenza viruses that differed by single mutations.

With current techniques it is not easy to make comparative analysis of influenza viruses with single mutations in their genomes, and it is extremely difficult to identify factors that limit the reassortment process between two influenza genomes that have infected the same cell.

The study was performed using single stem cells cultivated to provide sufficient DNA for whole genome sequencing.

Samani and his colleagues analyzed more than 500,000 genetic variations (naturally occurring, single - nucleotide differences) spanning the genome in blood cells collected from almost 3,000 people.

«Unique genome architectures after fertilization in single - cell embryos.»

The team of researchers (from three continents) have discovered that the egg and sperm genomes that co-exist in the single - cell embryo or zygote have a unique structure compared to other interphase cells.

Because of this we were able to find differences in genome folding on the level of single cells: these cell - to - cell variations were missed in conventional Hi - C due to the averaging over millions of cells,» says Ilya Flyamer, former Vienna Biocenter (VBC) summer student and then Master student and one of the first authors of the study.

Using a newly developed method researchers at the Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA) have been able to shed light on the complexity of genome reorganization occurring during the first hours after fertilization in the single - cell mammalian embryo.

HM - SNS allows researchers to sequence the genomes of single tumor cells and study multiple cells simultaneously, both lowering the cost and boosting data analysis for such studies.

Existing technology, known as next - generation sequencing (NGS), measures genomes derived from millions of cells versus the newer method for single - cell sequencing, called Monovar.

In addition, NIH - funded researchers who generate full genome sequence data from HeLa cells will be expected to deposit their data into a single database for future sharing through this process.

Using this method, features of genomic organization including compartments, topologically associating domains (TADs) and chromatin loops were detected in single cells when averaged over the genome.

«Single - cell analysis of embryos reveals mis - segregation of parental genomes.»

By sequencing the genome of the extensively studied moss Physcomitrella patens and comparing it to the sequenced genomes of rice, the flowering plant Arabidopsis, and single - cell algae, an international team has been able to look at what the ancestral land - plant genome looked like.

Here they photographed organisms from deep - water samples and used technology to sequence the genomes of these single - celled organisms.

Future optimization of recombination efficiencies achievable by SCRIBE could lead to more efficient single - cell digital memories and enhanced genome engineering technologies.

Whitehead Institute researchers have conducted the first genome - wide screen in Apicomplexa, a phylum of single - celled parasites that cause malaria, babesiosis, cryptosporidiosis, and toxoplasmosis.

The researchers gathered single cells of warnowiids off the coasts of B.C. and Japan, sequenced their genomes, and analyzed how the eyes are built using new methods in electron microscopy that allow the reconstruction of three dimensional structures at the subcellular level.

«This approach for data integration will enable the comparison of single - cell datasets and the ability to dissect the differences between them,» explains Rahul Satija, the study's senior author, who is an assistant professor in NYU's Center for Genomics and Systems Biology and a core faculty member at the New York Genome Center.

Prior to cell division, this DNA splits into two single strands, each bearing sequences of biochemical bases that form templates for the genomes of the daughter cells.

«And finally, it is much easier to follow a single mitochondrion and mitochondrial genome during cell division than to follow hundreds or even thousands at the same time,» Torsten Ochsenreiter from the Institute of Cell Biology scell division than to follow hundreds or even thousands at the same time,» Torsten Ochsenreiter from the Institute of Cell Biology sCell Biology says.

The assembly and analysis of human tumor cell genomes, many of which contain chromosome deletions, duplications and insertions, as well as single nucleotide changes, requires immense data storage capacity and high - speed computation.

However, through evolution, mammalian cells have developed a way to repair damaged bases in the single - stranded genome.

This includes the development of advanced laboratory tools such as CRISPR genome - editing and single - cell sequencing technologies.

Utilize turn - key analysis pipelines and visualization tools for investigating variation within whole genome, exome and single cell samples.

The most radical news at the conference was the first reported use of CRISPR / Cas9 to generate precise genome edits (changing only a single base pair) in two genes, not only in cell lines in vitro, but also in specific tissues of embryos in ovo.

SPAdes: a new genome assembly algorithm and its applications to single - cell sequencing.

«These extensions complement the MIxS suite of metadata standards by defining the key data elements pertinent for describing the sampling and sequencing of single - cell genomes and genomes from metagenomes,» said GSC President and study co-author Lynn Schriml of the Institute of Genome Sciences at University of Maryland School of Medicine.

This kit utilizes the DEF ‑ CS culture system, recognized for its suitability for genome engineering (Valton et al. 2014) and single - cell cloning (Feng et al. 2014), to promote reliable growth of hiPS cells in a feeder - free and defined environment.

«Starting 2007, the first single - cell genomes from environmental cells appeared in public databases and they are draft assemblies with fluctuations in the data quality.