Sentences with phrase «single cell imaging»

We also utilise a number of optical methods including single cell imaging and photo - activated ion channels such as Channelrhodopsins.

These techniques have been applied both for single molecule biophysics, single cell imaging and optical manipulation.

Not exact matches

It also includes an imaging station and CellSelect software, which images each nanowell and provides automatic selection of single cells for downstream processing.

«Imaging glucose uptake activity inside single cells.»

To overcome these problems, Min and his team developed a new modality to visualize glucose uptake activity inside single cells based on stimulated Raman scattering (SRS) imaging, and demonstrated its use in live cancer cells, tumor xenograft tissues, primary neurons and mouse brain tissues.

When the team inserted the new analogues into a living cell and used high - definition, single fluorescent - molecule imaging, they were finally able to directly document the actions of specific gangliosides in a living cell for the first time.

They married a 5 - foot - tall superconducting resonance imaging magnet to an optical microscope to observe a single cell suspended in a narrow, transparent chamber.

The group combines several cutting - edge single molecule imaging techniques to study how protein organization, dynamics and stoichiometry relate to protein function in several fundamental biological processes, such as intracellular transport, autoimmune neurological disorders or stem cell reprogramming.

He's also eager to return full time to his HHMI - supported lab at the University of California, Berkeley, which has shifted its work on gene regulation from wet biochemistry to imaging single molecules in living cells.

The researchers used a 2 - photon photolysis technique that can be performed in vivo, together with imaging, to manipulate and monitor neuronal activity at single - cell resolution.

Systems Biology and Genomics, including systems neurobiology, quantitative cell biology, cellular dynamics, algorithms, methods and technology development, data integration and visualization, imaging, synthetic biology, deep learning applied to biology and human health, and single cell biology.

But these imaging data are represented in completely different formats, and there's no way to switch between the two: once scientists zoom in to the level of single cells, they can not pan out again to see those cells in the context of the whole brain.

Currently the LifeTime consortium consists of over 60 leading single - cell biologists, computer scientists, mathematicians, clinicians, pathologists, imaging experts, and physicists from over 50 European institutions in 18 countries.

To answer this question, a study presented at the AACR Annual Meeting 2018 used real - time, single - cell imaging to make movies of live cancer cells.

Their findings in human cells complement and extend similar findings made recently in other organisms,» said Arjun Raj, PhD, assistant professor of bioengineering at the University of Pennsylvania and an expert in imaging single molecules within cells.

diSPIM technology enables rapid 3D imaging of samples ranging from single cells to small organisms over the course of hours to days.

Bethesda, USA (2016 - present) Research areas: Super-resolution microscopy, single - molecule imaging, gene expression, computational modeling and data analysis This section includes all projects during my postdoctoral research stay at the National Institutes of Health in Bethesda, MD (Unites States): (9) Understanding gene expression in eukaryotic cells»

In particular, we want to decipher the impact of mitochondrial dynamics and ultra-structure on OXPHOS functionality and mitochondrial performance by imaging the relevant processes on the single organelle and single molecule level in human cells.

For the type I interferon (IFN) receptor (IFNAR) as a paradigm for functional plasticity, we have, within the first funding period, established single molecule imaging techniques for monitoring receptor assembly and dynamics in the plasma membrane of living cells.

Multi-dimensional functional data were generated and analyzed through cutting - edge techniques, such as CyTOF, single - cell RNAseq, and high - content imaging.

The Calgary Microscopy and Imaging Facility (MIF) is a world - class university - wide facility housing transmission electron microscopy (TEM), scanning electron microscopy (SEM), advanced light microscopy, atomic force microscopy (AFM), including single cell force spectroscopy (SCFS), and advanced image processing for three - dimensional electron and light microscopy, directed by Professor Matthias Amrein.

Using single - molecule imaging, super-resolution microscopy and various biophysical and molecular approaches she will explore how gene expression in living cells works.»

7 Imaging Gene Expression in Living Cells at the Single - Molecule Level.

Ultimately, the combination of this new method of focusing on gene activity in single cells with other single - cell techniques involving microscopic imaging is likely to reveal the origins of developmental disorders of the brain, he added.

To this end, we are developing a new multi-scale live imaging approach to characterize nuclear organization dynamics at the single cell level based on the simultaneous characterization of both the local dynamics of RNA polymerases and transcription factors and the large scale behavior of chromatin.

She develops techniques and methodologies based on optical imaging, bio-sensing and single - molecule manipulation with the aim to monitor the behavior of individual biological molecules and compelxes in vitro and in live cells.

Single - Molecule Imaging of RNA Splicing in Live Cells.

This research makes use of single - molecular fluorescence imaging technology to visualize and analyze the behavior of exogenous DNA that were introduced into the cell from outside.

But the development of new, high - resolution imaging technology able to capture single cells has finally enabled scientists to reliably chart noise at this level, and to study how it influences behavior.

We are using live cell imaging, single cell analysis and gene editing to resolve the molecular determinants of human epidermal progenitor cell division outcomes (two progenitors, two differentiating cells or one or each) and the mode of cell division (maintenance mode vs repair mode).

Our multidisciplinary approach includes transgenesis, real time imaging, multi-parameter flow cytometry, transcriptomics, RNA interference, proteomics and single cell methodologies.

We focus on squamous tissues, the skin epidermis and the lining of the oesophagus, using transgenic models, novel sequencing approaches, live imaging and single cell analysis to uncover key steps in cancer development, with aim of developing rational interventions to decrease cancer risk.

Furthermore, the microscope will be capable of performing live - cell super-resolution imaging through structured illumination microscopy (SIM) and Super-Resolution Radial Fluctuations (SRRF); for fixed cells resolutions on the scale of tens of nanometres will be achievable using single molecule localization microscopy (SMLM) techniques.

Foreign researchers honored as the 31st KIA laureates include: Prof. Eric Vivier (France) for his research on harnessing innate immunity against cancer; Prof. Jianfang Wang (Hong Kong, China) for his research on Colloidal Plasmonic Metal Nanocrystals; Prof. Majed Chergui (Switzerland) for his research on unravelling the fundamentals of solar; Prof. Katharina Gaus (Australia) for her research on Single molecule imaging of T cell receptor signaling; and Prof. Dr. Burkhard Büdel (Germany) for his research on Role of lichens and cyanobacteria in biological soil crusts.

・ Use of single - molecular fluorescence imaging technology to elucidate the fate of DNA introduced into a living cell.

For a systems - level understanding of all crucial protein interactions during cell division, we are combining automated single molecule calibrated imaging and computational data analysis with advanced machine learning and modelling approaches to build an integrated protein atlas of the human dividing cell.

Being able to isolate, manipulate and image single & multiple cells in their natural environments is vital to researchers in many fields, notably those involved in drug discovery, early stage diagnosis and cellular force interactions making the addition of FluidFM ® to the world of AFM imaging and force spectroscopy an ideal pairing.

This article describes methods for preparation and imaging of 3D cancer cell cultures, either as single cells or spheroids.

These versatile tools enable quantitative, functional imaging of molecular interactions, protein activities or second messenger concentration in single living cells.

To pursue these studies, we employ Hi - C and ChIA - PET genomic technologies and single locus / live - cell imaging approach.

To pursue these studies, the Noma laboratory employs genomic technologies and single locus / live - cell imaging technology along with molecular and chromatin biology and epigenetics.

Generation of dispersed presomitic mesoderm cell cultures for imaging of the zebrafish segmentation clock in single cells.

While PALM is typically limited to a single read - out cycle, life cell dSTORM opens exciting possibilities for superresolution imaging of cellular nanostructures over prolonged time scales (1).