Sentences with phrase «single cell marker»

«We couldn't have figured out what was happening without the expertise of our collaborators at U.C. Davis, who determined the genetic sequence of every single cell marker on the cells in the lymph nodes,» says Miller.

Not exact matches

The cells are tiny — a few could fit on the head of a pin — so a single biodrone could carry several, enabling it to measure multiple atmospheric markers simultaneously.

At this time, it is only possible to verify the decision retrospectively with cell surface markers,» explains Dr. Carsten Marr, head of the Quantitative Single Cell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (Icell surface markers,» explains Dr. Carsten Marr, head of the Quantitative Single Cell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (ICell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (ICB).

Two additional panels will be established allowing more than 60 different immune markers to be monitored at the single - cell level, followed by data analysis using SB tools available at CEA.

Quantitative proteomics study of breast cancer cell lines isolated from a single patient: discovery of TIMM17A as a marker for breast cancer.

Title: Single - molecule transcript counting of stem - cell markers in the mouse intestine Authors: Itzkovitz S, Lyubimova A, Blat IC, Maynard M, van Es J, Lees J, Jacks T, Clevers H, van Oudenaarden A Date: 2011 Publication Details: Nat Cell Bcell markers in the mouse intestine Authors: Itzkovitz S, Lyubimova A, Blat IC, Maynard M, van Es J, Lees J, Jacks T, Clevers H, van Oudenaarden A Date: 2011 Publication Details: Nat Cell BCell Biol.

Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.

Now, in a study published in Stem Cells, the laboratory of Seung Hyun Kim (Hanyang University, Seoul, Korea) report on an open ‐ label, single ‐ arm, investigator ‐ initiated trial to identify biological markers of MSCs to predict response to in patients with ALS [3].

Our facility houses two third - generation Helios mass cytometers that can routinely analyze more than 40 markers per tube to provide an unprecedented «systems - level» view of phenotypic and functional variations among single cells within heterogeneous cell populations.

Three recent experimental studies focused on low consumption / exposure.949596 In one study, 29 smokers each consumed a single cigarette, immediately after which they had a significant decrease in blood vessel output power and significant increase in blood vessel ageing level and remaining blood volume 25 minutes later, as markers of atherosclerosis.94 In another study, human coronary artery endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation of oxidant stress sensing transcription factor NFR2 and up - regulation of cytochrome p450, considered to have a role in the development of heart disease.95 These effects were not seen when heart cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain cells.96

Our analysis of 23,184 cells identifies 15 clusters, few of which could be fully characterised by a single marker gene.

(A) Expression of 5 stem marker genes in single cells isolated from the edge (E), mid (M) or adjacent center (C) region of HES2 colonies.

(B) Percent of sorted single HES3 cells in (A) expressing stem or lineage markers according to level of surface marker expression.

Expression of four stem cell marker genes normalized to cyclophilin in nine single cell equivalents prepared from a pool of nine lysed HES3 cells.

Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein - tagged cells, assessing apoptosis and cell cycle status, the identification / isolation of stem and cancer stem cells (e.g., marker positive, side - population, etc.) and the deposition of single cells.

Single cell Q - RTPCR for the genes indicated was carried out on cells separated by flow cytometry on the basis of cell surface marker expression.

Internalization of POS is observed in a single optical y - axis projection (< 1 µm) of pigmented iPS - RPE cells labelled with the apical cell surface marker ATP1B1 (red).

(B) Percent of isolated single cells expressing stem or lineage markers according to location with the colony.

New computational strategies are supported by our single cell data analysis experts with computational methods deconvolute tissue composition, determine cell type heterogeneity, identify novel cell type defining markers or track transcriptional dynamics.

His current research interests include developing morphologic and biologic markers of embryo viability in order to enable single embryo transfer and avoid multiple pregnancies; optimizing preimplantation genetic testing; and deriving human embryonic stem cell lines.